Abstract
We investigated the anaerobic ammonium oxidation (anammox) reaction in a labscale upflow anaerobic sludge blanket (UASB) reactor. Our aim was to detect and enrich the organisms responsible for the anammox reaction using a synthetic medium that contained low concentrations of substrates (ammonium and nitrite). The reactor was inoculated with granular sludge collected from a full-scale anaerobic digestor used for treating brewery wastewater. The experiment was performed during 260 days under conditions of constant ammonium concentration (50 mg NH4/+-N/L) and different nitrite concentrations (50∼150 mg NO2-N/L). After 200 days, anammox activity was observed in the system. The microorganisms involved in this anammox reaction were identified as CandidatusB. Anammoxidans andK. Stuttgartiensis using fluorescencein situ hybridization (FISH) method.
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Thuan, TH., Jahng, DJ., Jung, JY. et al. Anammox bacteria enrichment in upflow anaerobic sludge blanket (UASB) reactor. Biotechnol Bioproc E 9, 345–351 (2004). https://doi.org/10.1007/BF02933055
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DOI: https://doi.org/10.1007/BF02933055