Abstract
A structured kinetic model was proposed to describe cell growth and synthesis of a secondary metabolite, berberine, in batch suspension culture ofThalictrum rugosum. The model was developed by representing the physiological state of the cell in terms of the activity and the viability, which can be estimated using the culture fluorescence measurement. In the proposed model, the cells were divided into three types; active-viable, nonactive-viable, and dead cells. The model was formulated in terms of cell growth (dry/fresh weight, activity, and viability), carbon source utilization (sucrose, glucose and fructose), and product formation (intracellular and extracellular berberine). The concept of cell expansion and the death phase were also included in this model to describe the sugar accumulation and the release of intracellular berberine into medium by cell lysis, respectively. The parameters used in this model were estimated based on the experimental results in conjunction with numerical optimization techniques. Satisfactory agreement between the model and experimental data was obtained. The proposed model could accurately predict cell growth and product synthesis as well as the distribution of the secondary metabolite between the cell and the medium. It is suggested that the proposed model could be extended as a useful framework for quantitative analysis of physiological characteristics in the other plant cell culture systems.
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Abbreviations
- A :
-
activity (g/g)
- FI :
-
relative fluorescence intensity
- k :
-
rate constant (day−1)
- K :
-
Monod constant (g/L)
- P :
-
product concentration (g/L)
- S :
-
substrate concentration (g/L)
- t :
-
time (days)
- V :
-
viability (g/g)
- X :
-
biomass concentration (g/L)
- Y :
-
yield coefficient (g/g)
- α:
-
growth-associated production constant (g/g/day)
- β:
-
nongrowth-associated production constant (g/g/day)
- κ:
-
cell expansion coefficient (day−1)
- μ:
-
specific growth rate (day−1)
- λ:
-
product degradation constant (g/g/day)
- γ:
-
product release coefficient by cell lysis (g/g)
- θ:
-
function for cell expansion
- ϕ:
-
function for activity loss
- c:
-
conversion from sucrose
- d:
-
dry weight or death
- E:
-
extracellular
- f:
-
fresh weight
- F:
-
fructose
- G:
-
glucose
- I:
-
intracellular
- L:
-
lag phase
- m:
-
maximum
- o:
-
initial
- S:
-
sucrose
- t:
-
total
- ad:
-
active-viable cell
- dd:
-
dead cell
- nd:
-
nonactive-viable cell
- vd:
-
viable cell
- P/SF :
-
product from fructose
- P/SG :
-
product from glucose
- X/SF :
-
biomass from fructose
- X/SG :
-
biomass from glucose
References
Prenosil, J. E. and H. Pedersen (1983) Immobilized plant cell reactors.Enzyme Microb. echnol. 5: 323–331.
Panda, A. K., S. Mishira, V. S. Bisaria, and S. S. Bhojwani (1989) Plant cell reactors—a perspective.Enzyme Microb. Technol. 11: 386–397.
Drapeau, D., H. W. Blanch, and C. R. Wilke (1986) Growth kinetics ofDioscorea deltoidea andCatharanthus roseus in batch culture.Biotechnol. Bioeng. 28: 1555–1563.
Schnapp, S. R., W. R. Curtis, R. A. Bressan, and P. M. Hasegawa (1991) Estimation of growth yield and maintenance coefficient of plant cell suspensions.Biotechnol. Bioeng. 38: 131–1136.
Shimogawara, K. and H. Usuda (1995) Uptake of inorganic phosphate by suspension-cultured tobacco cells: kinetics and regulation by Pi starvation.Plant Cell Physiol. 36: 341–351.
Takeda, T., Y. Takeuchi, M. Seki, S. Furusaki, and H. Matsuoka (1998) Kinetic analysis of cell growth and vitamin E production in plant cell culture ofCarthamus tinctorius using a structured model.Biochem. Eng. J. 1: 233–242.
Frazier, G. C. (1989) A simple, leaky cell growth model for plant cell aggregates.Biotechnol. Bioeng. 33: 313–320.
Bailey, C. M. and H. Nicholson (1989) A new structured model for plant cell culture.Biotechnol. Bioeng. 34: 1331–1336.
De Gunst, M. C. M., P. A. A. Harkes, J. Val, W. R. van Zwet, and K. R. Libbenga (1990) Modeling the growth of a batch culture of plant cells: a corpuscular approach.Enzyme Microb. 12: 61–71.
Bramble, J. L., D. J. Graves, and P. Brodelius (1991) Calcium and phosphate effects on growth and alkaloid production inCoffea arabica: experimetal results and mathematical model.Biotechnol. Bioeng. 37: 859–868.
Hooker, B. S. and J. M. Lee (1992) Application of a new structured model to tobacco cell culture.Biotechnol. Bioeng. 39: 765–774.
Zabriskie, D. W. and A. E. Humphrey (1978) Estimation of fermentation biomass concentration by measuring culture fluorescence.Appl. Environ. Microbiol. 33: 337–343.
Edelman, J. and A. D. Hanson (1971) Sucrose suppression of chlorophyll synthesis in carrot tissue culture: the role of invertase.Planta 101: 122–132.
Fowler, M. W. (1982) Substrate utilization by plant-cell culture.J. Chem. Tech. Biotechnol. 32: 338–346.
Metzler, C. M., G. L. Elfring, and A. J. McEwen (1974) A package of computer programs for pharmaco-kinetic modeling.Biometrics 30: 562–563.
Glicklis, R., D. Mills, D. Sitton, W. Stortelder, and J. C. Merchuk (1998) Polysaccharide production by plant cells in suspension: experiments and mathematical modeling.Biotechnol. Bioeng. 57: 732–740.
Cazzulino, D. L., H. Pedersen, C. K. Chin, and D. Styer (1990) Kinetics of carrot somatic embryo development in suspension culture.Biotechnol. Bioeng. 35: 781–786.
Kanabus, J., R. A. Bressan, and N. C. Carpita (1986) Carbon assimilation in carrot cells in liquid culture.Plant Physiol. 82: 363–368.
Harrison, D. E. F. and B. Chance (1970) Fluorimetric technique for monitoring changes in the level of reduced nicotinamide nucleotides in continuous culture of microorganisms.Appl. Microbiol. 19: 446–450.
Zenk, M. H., M. Rueffer, M. Amann, B. Deus-Neumann, and N. Nagakura (1985) Benzyl-isoquinoline biosynthesis by cultivated plant cells and isolated enzyme.J. Nat. Prod. 48: 725–738.
Seinfeld, J. and L. Lapidus (1970)Process Modeling Estimation and Identification Prentice-Hall Inc. Englewood Cliffs, NJ, U.S.A.
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Choi, JW., Kim, YK., Lee, W.H. et al. Kinetic model of cell growth and secondary metabolite synthesis in plant cell culture ofThalictrum rugosum . Biotechnol. Bioprocess Eng. 4, 129–137 (1999). https://doi.org/10.1007/BF02932383
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DOI: https://doi.org/10.1007/BF02932383