Abstract
Three isolates ofPseudomonas aeruginosa were used for seed treatment of rice; all showed plant growth promoting activity and induced systemic resistance in rice againstRhizoctonia solani G5 and increased seed yield. Production of salicylic acid (Sal) byP. aeruginosa bothin vitro andin vivo was quantified with high performance liquid chromatography. All three isolates produced more Sal in King’s B broth than in induced roots. Using a split root system, more Sal accumulated in root tissues of bacterized site than in distant roots on the opposite site of the root system after 1 d, but this difference decreased after 3 d. Sal concentration 0–200 g/L showed no inhibition of mycelial growth ofR. solani in vitro, while at ≥300 g/L it inhibited it.P. aeruginosa-pretreated rice plants challenged inoculation withR. solani (as pathogen), an additional increase in the accumulation of peroxidase was observed. Three pathogenesis-related peroxidases in induced rice plants were detected; molar mass of these purified peroxidases was 28, 36 and 47 kDa. Purified peroxidase showed antifungal activity against phytopathogenic fungiR. solani, Pyricularia oryzae andHelminthosporium oryzae.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- CD:
-
critical difference
- HPLC:
-
high performance liquid chromatography
- IAA:
-
3-indolylacetic acid
- ISR:
-
induced systemic resistance
- PDA:
-
potato-dextrose agar
- PGPR:
-
plant growth promoting rhizobacteria
- PR:
-
pathogenesis-related
- Sal:
-
salicylic acid
- SAR:
-
systemic acquired resistance
- ShB:
-
sheath blight
- TMV:
-
tobacco mosaic virus
References
Audenaert K., Pattery T., Cornelis P., Hofte M.: Induction of systemic resistance toBotrytis cinerea in tomato byPseudomonas aeuginosa 7NSK2: role of salicylic acid, pyochelin and pyocyanin.Mol.Plant-Microbe Interact.15, 147–156 (2002).
Beffa R., Martin H.V., Pilet P.E.:In vitro oxidation of indole acetic acid by soluble auxin-oxidases and peroxidase from maize roots.Plant Physiol.94, 485–491 (1990).
Benhamou N., Gagne S., Quere D.L., Debhi L.: Bacterial-mediated induction resistance in cucumber: beneficial effect of the endophytic bacteriumSerratia phymuthica on the protection against infection byPythium ultimum.Phytopathology90, 45–56 (2000).
Buysens S., Heungens K., Poppe J., Hofte M.: Involvement of pyochelin and pyroverdin in suppression ofPythium-induced damping-off tomato byPseudomonas aeruginosa 7NSK2.Appl.Environ.Microbiol.62, 865–871 (1996).
Chen C., Belanger R., Benhamou N., Paulitz T.C.: Role of salicylic acid in systemic resistance induced byPseudomonas spp. againstPythium aphanidermatum.Eur.J.Plant Pathol.105, 477–486 (1999).
Chittoor J.M., Leach R.E., White F.F.: Differential induction of peroxidase gene family during infection of rice byXanthomonas oryzae pv.oryzae.Mol.Plant Microbe Interact.10, 861–871 (1997).
Goldberg R., Imberty A., Liberman M., Prat R.: Relationships between peroxidatic activities and cell wall plasticity, pp. 208–220 inMolecular and Physiological Aspects of Plant Peroxidases (H. Greppin, C. Peneland, T. Gaspar, Eds). University of Geneva, Geneva (Switzerland) 1986.
Grisebach H.: Lignins, pp. 457–478 inThe Biochemistry of Plants, Vol. 7 (E.E. Conn, Ed.). Academic Press, New York 1981.
Hammerschmidt R., Nuckles F., Kuc I.: Association of enhance peroxidase activity with induced systemic resistance of cucumber toColletotrichum largenarium.Physiol.Plant Pathol.20, 73–82 (1982).
Knoester M., Pieterse C.J.M., Bol J.F., Van Loon L.C.: Systemic resistance inArabidopsis induced by rhizobacteria requires ethylene-dependent signaling at the site of application.Mol.Plant-Microbe Interact.12, 720–727 (1999).
Maijala P., Harrington T.C., Raudaskoski M.: A peroxidase gene family and gene treesHeterohasidion and related genera.Mycologia95, 209–221 (2003).
Malamy J., Leon J.P., Klessig D.F., Raskin I.: Salicylic acid: a likely endogenous signal in the resistance response of tobacco to viral infection.Science250, 1002–1004 (1990).
Manandhar H.K., Mathur S.B., Smedegaard-Petersen V., ThordalChristensen H.: Accumulation of transcripts for pathogenesis related proteins and peroxidase in rice plant triggered byP. oryzae, Bipolaris sorokiniana and UV light.Physiol.Mol.Plant Pathol.55, 289–295 (1999).
Meena B., Marimuthu T., Velazhahan R.: Salicylic acid induced resistance in groundnut against late leaf spot caused byCercoaporidium personatum.J.Mycol.Plant Pathol.31, 139–145 (2001).
Metraux J.P., Singer H., Ryals J., Ward E., Wyss-Benz M., Gaudin J., Raschdorf K., Schmid E., Blum W., Inverardi B.: Increase in salicylic acid at the onset of systemic acquired resistance in cucumber.Science250, 1004–1006 (1990).
Meyer G.D., Hofte M.: Salicylic acid produced by the rhizobacteriumPseudomonas aeruginosa 7NSK2 induced resistance to leaf infection byBotrytis cinerea on bean.Phytopathology87, 587–593 (1997).
Ou S.H.:IRRI Annual Report 1967, p. 40. Banos, Philippines 1973.
Pan S.Q., Ye X.S., Kuc I.: A technique for detection of chitinases, β-1,3-glucanases and protein patterns, after single separation using PAGE or isolectric focusing.Phytopathology81, 970–974 (1991).
Park S., Kloepper J.W.: Activation of PR-1a promoter by rhizobacteria that induced systemic resistance in tobacco againstPseudomonas syringae pv.tabaci.Biol.Control18, 2–9 (2000).
Pieterse C.M.J., Van Loon L.C.: Salicylic acid-independent plant defense pathways.Trends Plant Sci.4, 52–58 (1999).
Pieterse C.M.J., Van Wees S.C.M., Hoffland E., Van Pelt J.A.: Systemic resistance inArabidopsis induced by biocontrol bacteria is independent of salicylic acid accumulation and pathogenesis-related gene expression.Plant Cell8, 1225–1237 (1996).
Ramamoorthy V., Samiyappan R.: Induction of defense-related genes inPseudomonas fluorescens-treated chilli plants in response to infection byColletotrichum capsici.J.Mycol.Plant Pathol.31, 146–155 (2001).
Ramanathan A., Vidhyasekaran P., Samiyappan R.: Two pathogenesis-related peroxidases in greengram (Vigna radiata (L.)Wilczek) leaves and cultured cells induced byMacrophomina phaseolina (Tassi)Goid, and its elicitor.Microbiol.Res.156, 139–144 (2001).
Roy A.K.: Susceptibility of rice plant sheath blight at different stages of growth.Kavaka7, 25–26 (1979).
Saikia R., Singh T., Kumar R., Srivastava J., Srivastava A.K., Singh K., Arora D.K.: Role of salicylic acid in systemic resistance induced byPseudomonas fluorescens againstFusarium oxysporum f.sp.ciceri in chickpea.Microbiol.Res.158, 203–213 (2003).
Saikia R., Kumar R., Singh T., Srivastava A.K., Arora D.K., Lee M.-W.: Induction of defense related enzymes and pathogenesis related proteins inPseudomonas fluorescens-treated chickpea in response to infection byFusarium oxysporum f.sp.ciceri.Mycobiology32, 47–52 (2004).
Saikia R., Singh B.P., Kumar R., Arora D.K.: Detection of pathogenesis related proteins — chitinase and β-1,3-glucanase in induced chickpea.Curr.Sci.89, 659–663 (2005).
Saparrat M.C.N., Guillén F.: Ligninolytic ability and potential biotechnology applications of the South American fungusPleurotus laciniatocrenatus.Folia Microbiol.50, 155–160 (2005).
Schmid P.S., Feucht W.: Tissues-specific oxidation browning of polyphenols by peroxidases in cherry shoots.Gartenbauwissenschaft45, 68–73 (1980).
Silverman P., Seskar M., Kanter D., Schweizer P., Metraux J.P., Raskin I.: Salicylie acid in rice: biosynthesis, conjugation and possible role.Plant Physiol.108, 633–639 (1995).
Van Loon L.C.: Systemic induced resistance, pp. 521–574 inMechanisms of Resistance to Plant Diseases (A. Slusarenko, R.S.S. Fraser, L.C. Van Loon, Eds.). Kluwer Academic Publisher, Dordrecht 2000.
Van Loon L.C., Pierpoint W.S., Boller T., Conejero V.: Recommendation for naming plant pathogenesis related proteins.Plant Mol.Biol.Res.12, 245–264 (1994).
Van Loon L.C., Bakker P., Pieterse C.M.J.: Systemic resistance induced by rhizosphere bacteria.Ann.Rev.Phytopathol.36, 453–483 (1998).
Vernooji B., Friedrich L., Morse A., Reist R., Kolditz-Jawhar R., Ward E., Ukness S., Kessmann H., Ryals J.: Salicylic acid is not the translocated signal responsible for inducing systemic acquired resistance but it required in signal transduction.Plant Cell6, 959–965 (1994).
Víteček J., Kašparovský T., Mikešová M., Mikeš V.: Nonspecific elicitation of defense reaction in suspension tobacco cells by elicitors fromArmillaria.Folia Microbiol.50, 128–132 (2005).
Ward E., Uknes S.J., Williams S.C., Dincher S.S., Wiederhold D.L., Alexander D.C., Ahl-Goy P., Metraux J.P., Ryals J.: Coordinate gene activity in response to agents that induce systemic acquired resistance.Plant Cell3, 1085–1094 (1991).
Yalpani N., Silverman P., Wilson T.M.A., Kleier D.A., Raskin I.: Salicylic acid is a systemic signal and an inducer of pathogenesis-related proteins in virus-infected tobacco.Plant Cell3, 809–818 (1991).
Ye X.S., Pan S.Q., Kuc J.: Activity, isoenzyme pattern and cellular localization of peroxidase as related to systemic resistance of tobacto to blue mold (Peronospora tabacina) and to tobacco mosaic virus.Phytopathology80, 1295–1299 (1990).
Young S.A., Guo A., Guikema J.A., White F., Leach I.E.: Rice cationic peroxidase accumulation in xylem vessels during incompatible interactions withXathomonas oryzae pv.oryzae.Plant Physiol.107, 1333–1341 (1995).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Saikia, R., Kumar, R., Arora, D.K. et al. Pseudomonas aeruginosa inducing rice resistance againstRhizoctonia solani: Production of salicylic acid and peroxidases. Folia Microbiol 51, 375–380 (2006). https://doi.org/10.1007/BF02931579
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02931579