Abstract
Ferromagnetic Dacron (polyethyleneterephthalate) is proposed as a matrix to immobilize proteins covalently. Dacron in powder was magnetized by reacting ferrous (Fe+2) and ferric (Fe+3) ions with its hydrazide form at pH 8.3. Ferromagnetic hydrazide Dacron was converted to ferromagnetic azide Dacron and amyloglucosidase (E.C. 3.2.1.3) was covalently bound through the latter group. The catalytic property of the enzyme was preserved (8% of the specific activity estimated for the soluble enzyme) and all the magnetic amyloglucosidase Dacron derivative was recovered by using a magnetic field. No activity was detected in the supernatant.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Weetall, H. H. (1970),J. Biomed. Mater. Res. 4, 597–599.
Goldstein, L., Freeman, A., Blassberger, D., Granot, R., and Sokolovsky. (1977),Biotechnological Applications of Proteins and Enzymes, Bohak, Z. and Sharon, N., eds., New York, pp. 156-159.
Melo, E. H. M. (1984), MS thesis, submitted to the Department of Biochemistry of Universidade Federal de Pernambnco, Brazil.
Carvalho, L. B., Jr., Melo, E. H. M., Vasconcelos, A. R. A., and Lira, R. R. (1986),Arquivos de Biologia e Tecnologia 29, 525–531.
Carvalho, L. B., Jr., Silva, M. P. C., and Melo, E. H. M. (1987),Brazilian J. Med. Biol. Res. 20, 521–526.
Oliveira, E. A., Silva, M. P. C., Figueiredo, Z. M. B., and Carvalho, L. B., Jr. (1989),Appl. Biochem. Biotechnol.22, 109–114.
Tamaura, Y., Takahashi, K., Kodera, Y., Saito, Y., and Inada, Y. (1986),Biotechnol. Lett. 8, 877–880.
Takahashi, K., Tamaura, Y., Kodera, Y., Mihama, T., Saito, Y., and Inada, Y. (1987),Biochem. Biophys. Res. Commun.142, 291–296.
Yoshimoto, T., Takeshi, M., Takahashi, K., Saito, Y., Tamaura, Y., and Inada, Y. (1987),Biochem. Biophys. Res. Commun.145, 908–914.
Inada, Y., Ohwada, K., Yoshimoto, T., Kojima, S., Takahashi, K., Kodera, Y., Matsushima, A., and Saito, Y. (1987),Biochem. Biopliys. Res. Commun.148, 392–396.
Inada, Y., Takahashi, K., Yoshimoto, T., Kodera, Y., Matsushima, A., and Saito, Y. (1988),TIBTECH 6, 131–134.
Bernfeld, P. (1955),Methods in Enzymology, Colowich, S. P. and Kaplan, N. O., eds., Academic, New York, pp. 149-150.
Eggstein, H. and Kreutz, F. H. (1955),Klin. Wochschr.33, 879–884.
Author information
Authors and Affiliations
Additional information
Author to whom all correspondence and reprint requests should be addressed.
Rights and permissions
About this article
Cite this article
Carneiro LeÃo, A.M.A., Oliveira, E.A. & Carvalho, L.B. Immobilization of protein on ferromagnetic Dacron. Appl Biochem Biotechnol 31, 53–58 (1991). https://doi.org/10.1007/BF02922125
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02922125