Abstract
The tribe Oryzeae consists of 12 genera and 71 species with a world distribution.Zizania latifolia (Griseb.) Turcz. ex Stapf is included in this tribe and possesses numerous traits valuable for rice breeding, such as disease and insect resistance, cold and flood tolerance, and high grain quality. The genetics and breeding ofZ. latifolia are still in their infancy. To facilitate genomic studies ofZizania, a genomic DNA library was constructed using a transformation-competent artificial chromosome (TAC) vector system. The TAC library contains 91, 584 TAC clones with an average insert size of approximately 45 kb, covering six haploidZizania genome equivalents. Very low signals after hybridization with chloroplast and mitochondrial genes indicate that the TAC library is predominantly composed of nuclear DNA. The TAC clones were stable inE. coli for 100 generations. Clones containing thedihydrodipicolinate synthase (DHPS) gene were screened by pooled PCR. The positive clones can be used forZ. latifolia DHPS gene cloning and functional analysis. The library will be useful in studies of genome structure, gene cloning and evolution of rice.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- CIAP:
-
calf intestinal alkaline phosphatase
- DHPS:
-
dihydrodipicolinate synthase
- HMW:
-
high molecular weight
- PFGE:
-
pulsed field gel electrophoresis
- TAC:
-
transformation-competent artificial chromosome
References
Adam-Blondon AF, Bernole A, Faes G, Lamoureux D, Pateryron S, Grando MS, Caboche M, Velzsco R, and Chalhoub B (2005) Construction and characterization of BAC libraries from major grapevine cultivars. Theor Appl Genet 110: 1363–1371.
Dereppe C, Bold G, Ghisalba O, Ebert E, Schar HP (1992) Purification and characterisation of dihydrodipicolinate synthase from pea. Plant Physiol 98: 813–821.
Fang YD, Liu YG, Wu H, Zhang QY, Chen PD, Liu DJ (2000) Construction of a transformation-competent artificial chromosome (TAC) library of a wheat-haynaldia villosa translocation line. Chinese J of Biotech 16: 433–436.
Gad Galili (1995) Regulation of lysine and threonine synthesis. The Plant Cell 7: 899–906.
Ghislain M, Frankard V, Jacobs M (1990) Dihydrodipicolinate synthase ofNicotiana sylvestris, a chloroplast localised enzyme of the lysine pathway. Planta 180: 480–486.
Hass BL, Pires JC, Porter R, Philips RL, Jackson SA (2003) Comparative genetics at the gene and chromosome levels between rice (Oryza sative) and wildrice (Zizania palustris). Theor Appl Genet 107: 773–782.
Kennard W, Philips R, Porter R, Grombacher A (1999) A comparative map of wild rice (Zizania palustris, L. 2n=2x=30). Theor Appl Genet 99: 793–799.
Kennard WC, Philips RL, Porter RA (2002) Genetic dissection of seed shattering, agronomic, and color traits in American wildrice (Zizania palustris var. interior L.) with a comparative map. Theor Appl Genet 105: 1075–1086.
Kumpaisal R, Hashimoto T, Yamada Y (1987) Purification and characterisation of dihydrodipicolinate synthase from wheat suspension cultures. Plant Physiol 85: 145–151.
Leroy T, Marraccini P, Dufour M, Montagnon C, Lashermes P, Sabau X, Ferreira LP, Jourdan I, Pot D, Andrade AC, Glaszmann JC, Vieira IGE and Piffanelli P (2005) Construction and characterization of a Coffea canephora BAC library to study the organization of sucrose biosynthesis genes. Theor Appl Genet 109: 225–230.
Liang FS, Zhang KC, Yu ZW, Wang B (2004) Construction, characterization, and screening of a transformation-competent artificial chromosome library of peach. Plant Mol Biol Rep 22: 1–12.
Liu B, Liu ZL, Li XW (1999) Production of a highly asymmetric somatic hybrid between rice and Zizania latifolia (Griseb): evidence for inter-genomic exchange. Theor Appl Genet 98: 1099–1103.
Liu YG, Liu H, Chen LT, Qiu WH, Zhang QY, Wu H, Yang CY, Wang ZH, Tian DS, Mei MT (2002) Development of new transformation-competent artificial chromosome vectors and rice genomic libraries for efficient gene cloning. Gene 282: 247–255.
Liu YG, Nagaki K, Fujita M, Kawaura K, Uozumi M, Ogihara Y (2000) Development of an efficient maintenance and screening system for large-insert genomic DNA libraries of hexaploid wheat in a transformation-competent artificial chromosome (TAC) vector. Plant J 23: 687–695.
Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D (1999) Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning Proc Natl Acad Sci USA 96: 6535–6540.
Sambrook J, Frisch E, and Maniatis T (1989), Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring harbor Laboratory, Cold Spring Harbor, New York.
Shen B, Wang DM, McIntyre CL, Liu CJ (2005) A ‘Chinese Spring’ wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome rigions. Theor Appl Genet. 111: 1489–1494.
Shizuya H, Birren B, Kim UJ, Mancino V, Slepak T, Tachiiri Y, Simon M (1992) Cloning and stable maintenance of 300-kilobase-pair fragments inEscherichia coli using an F-factor-based vector. Proc Natl Acid Sci USA 89: 8794–8797.
Still DW, Kovach DA, and Bradford KJ (1994) Development of Desiccation Tolerance during Embryogenesis in Rice (Oryza sativa) and Wild Rice (Zizania palustris) (Dehydrin Expression, Abscisic Acid Content, and Sucrose Accumulation). Plant Physiol 104: 431–438.
Zhai CK, Zhang XQ, Sun GJ, Lu CM and Jiang ZK (2000) Study on nutrition composition and protein quality of a Chinese wild ricce. J Hygiene Res 29: 375–378.
Zhang HB, Choi S, Woo SS, Jiang J, Li Z, Wing RA (1996) Construction and characterization of two rice bacterial artificial chromosome libraries from parents of a permanent recombinant inbred mapping population. Mol Breeding 2: 11–24.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Kong, F.N., Jiang, S.M., Shi, L.X. et al. Construction and characterization of a transformation-competent artificial chromosome (TAC) library ofZizania latifolia (Griseb.). Plant Mol Biol Rep 24, 219–227 (2006). https://doi.org/10.1007/BF02914060
Issue Date:
DOI: https://doi.org/10.1007/BF02914060