Abstract
DNA methylation is an epigenetic phenomenon associated with gene silencing in transgenic plants, retrotransposons and virus infection. Expression analysis of specific genes in Arabidopsis methylation mutants showed an association between DNA methylation and gene expression. To determine whether DNA methylation is associated with resistance to black Sigatoka (BS) andMycosphaerella fijiensis (MF), we used anin vitro assay of mesophyll cell suspensions of reference cultivars with known resistance to BS. Methylation of CCmGG sequences was evaluated by methylation-sensitive amplification polymorphism (MSAP) markers of reference cultivars and somaclonal variants to identify molecular markers associated with resistance to MF toxins and BS. Four MSAP markers were associated with resistance (MAR) to MF toxins. The MSAP markers show a high degree of sequence similarity with resistance gene analog and with retrotransposon sequences. The MSAP markers are useful as molecular indicators of tolerance to MF toxins and resistance to BS.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- AFLP:
-
amplified fragment length polymorphism
- BA:
-
N-6-benzyladenine
- BS:
-
black Sigatoka
- MAR:
-
methylation associated with resistance
- MAS:
-
methylation associated with susceptibility
- MF:
-
Mycosphaerella fijiensis
- MSAP:
-
methylation-sensitive amplification polymorphisms
- TGS:
-
transcriptional gene silencing
- SAMPL:
-
selective amplification of microsatellite polymorphic loci
- TI:
-
tolerance index
References
Bender J (2004) DNA methylation and epigenetics. Annu Rev Plant Biol 55: 41–68.
Chalhoub BA, Thibault S, Laucou V, Rameau C, Höfte H, and Cousin R (1997) Silver staining and recovery of AFLP amplification products on large denaturing polyacrylamide gels. BioTechniques 22: 216–220.
Doyle JJ and Doyle JL (1990) Isolation of DNA from small amounts of plants tissues. BRL Focus 12: 13–15.
Engelborghs I and Swennen R (1999) Fluorescent AFLPs to detect dwarfism. The Case of the Curare Enano (Musa spp.). In: International Symposium on the Cellular and Molecular Biology of Banana. Tower Road, Ithaca, New York. March 22–24. p 10.
Feinberg AP (2001) Methylation meets genomics. Nature Genetics 27: 9–10.
Fouré E (1982) Les cercosporioses du bananier et leurs traitements: Étude de la sensibilité variétale des bananiers et des plantains àM. fijiensis Morelet au Gabon. FRUITS 37: 749–771.
García E, Giménez C, Vidal M, Palacios G, and Haddad O (2001) CIEN BTA-03: A new somaclonal variant resistant to yellow Sigatoka: Biochemical, Genetic and Molecular Characterization and Agronomic Studies. Memorias del 2do Simposio Internacional en Biología molecular y Celular de Banano. INFOMUSA (PROMUSA) p 11–13.
Giménez C and Colmenares M (2004) Evaluación in vitro de la resistencia a las toxinas deMycosphaerella fijiensis enMusa spp. Revista Ciencia FEC 12: 251–257.
Giménez C, Palacios G, Colmenares M, and Günter K (2005) SAMPL, a technique for somaclonal variation fingerprinting inMusa. Plant Mol Biol Rep 23: 263–269.
Hirohiko H, Hiroyuki O, and Tetsuji K (2000) Silencing of retrotransposons in Arabidopsis and reactivation by the ddm1 mutation. Plant Cell 12: 357–369.
Jacobsen SE, Sakai H, Finnegan EJ, Cao X, and Meyerowitz E (2000) Ectopic hypermethylation of flower-specific genes inArabidopsis. Curr Biol 10: 179–186.
Matthes M, Singh R, Cheah SC, and Karp A (2001) Variation in oil palm (Elaeis guineensis Jacq.) tissue culture-derived regenerants revealed by AFLPs with methylation-sensitive enzymes. Theor Appl Genet 102: 971–979.
Palacios G, Bustamante S, Molina C, Winter P, and Kahl G (2002) Electrophoretic identification of new genomic profiles with a modified selective amplification of microsatellite polymorphic loci technique based on AT/AAT polymorphic repeats. Electrophoresis 23(19): 3341–3345.
Peraza-Echeverria S, Herrera-Valencia VA, and James-Kay A (2001) Detection of DNA methylation changes in micropropagated banana plants using methylation-sensitive amplification polymorphism (MSAP). Plant Sci 161: 359–367.
Reyna-López GE, Simpson J, and Ruiz-Herrera J (1997) Differences in DNA methylation patterns are detectable during the dimorphic transition of fungi by amplification of restriction polymorphisms. Mol Gen Genet 253: 703–710.
Trevor LS and Richards EJ (2000) Mum’s the word: MOM and modifiers of transcriptional gene silencing. Plant Cell 12: 1003–1006.
Vos P, Hogers R, Bleeker M, Rijans M, Van der Lee T, Hormes M, Frijters A, Pot L, Peleman J, Kuiper M, and Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407–4414.
Weising K and Kahl G (1997) Hybridization-based microsatellite fingerprinting of plants and fungi. In: DNA Markers: Protocols, Applications and Overviews (eds. Caetano-Anollés G, Gresshoff P), pp 27–54. Wiley & Sons, New York.
Wessler SR, Bureau TE, and White SE (1995) LTR-retrotransposons and MITEs: Important players in the evolution of plant genomes. Curr Opin Genet Dev 5: 814–821.
Xiong LZ, Xu CG, Saghai MM, and Zhang Q (1999) Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique. Mol Gen Genet 261(3): 439–446.
Xu M, Li X, and Korban SS (2000) AFLP-based detection of DNA methylation. Plant Mol Biol Rep 18: 361–368.
Yuko W (2005) Physiological functions of plant DNA methyltransferases. Plant Biotech 22(2): 71–80.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Giménez, C., Palacios, G. & Colmenares, M. Musa methylated DNA sequences associated with tolerance toMycosphaerella fijiensis toxins. Plant Mol Biol Rep 24, 33–43 (2006). https://doi.org/10.1007/BF02914044
Issue Date:
DOI: https://doi.org/10.1007/BF02914044