Abstract
Anin vitro procedure for large scale multiplication ofBoswellia serrata Roxb. has been developed using cotyledonary node segments. In average 4 shoots per node were obtained on Murashige and Skoog's (MS) medium containing 0.5 mg dm−3 6-benzylaminopurine (BAP) and 0.05 mg dm−3 napthaleneacetic acid (NAA) within 22 d. By repeated subculture technique 90–100 shoots per node could be obtained after 88 d of initial culture. Shoots could be rooted on MS medium containing 1/4 salts, 1% saccharose, and a combination of 0.5 mg dm−3 indole-3-butyric acid (IBA) and 0.25 mg dm−3 indole-3-acetic acid (IAA). Addition of antioxidants like polyvinylpyrrolidone (PVP-50 mg dm−3) and ascorbic acid (100 mg dm−3) in both multiplication and rooting media prevented browning of cultures. Approximately 80% of shoots rooted within 8–10 d. Rooted plantlets were kept for 15 d in culture bottles containing SoilriteTM irrigated with a nutrient solution containing 1/4 MS salts and finally transferred to pots containing soil: SoilriteTM (1∶1), mixture with 70% transplantation success.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Abbreviations
- BAP:
-
6-benzylaminopurine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- Kn:
-
kinetin
- MS:
-
Murashige and Skoog's (1962) medium
- NAA:
-
napthaleneacetic acid
- PVP:
-
polyvinylpyrrolidone
References
Aitken-Christie, J., Connett, M.: Micropropagation of forest trees.—In: Kurata, K., Kozai, T. (ed.): Transplant Production Systems. Pp. 163–194. Kluwer Academic Publishers, Dordrecht-Boston-London 1992.
Hu, C.Y., Wang, P.J., Meristem, shoot tip and bud cultures.—In: Evans, D.A., Sharp, R.W., Ammirato, P.V., Yamada, Y. (ed.): Handbook of Plant Cell Culture. Vol. 1. Pp. 177–227. Macmillan, New York 1983.
Joshi, R.:In vitro studies on forest plants of Rajasthan.—Ph.D. Thesis, University of Jodhpur, Jodhpur 1991.
Mascarenhas, A.F., Muralidharan, E.M.: Tissue culture of forest trees in India.—Curr. Sci.58: 606–613, 1989.
McComb, J.A., Newton, S.: Propagation of Kangaroo Paws using tissue culture.—J. hort. Sci.56: 181–183, 1981.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.—Physiol. Plant.15: 473–497, 1962.
Rathore, T.S., Singh, R.P., Shekhawat, N.S.: Clonal propagation of desert teak (Tecomella undulata) through tissue culture.—Plant Sci.79: 217–222, 1991.
Rathore, T.S., Deora, N.S., Shekhawat, N.S.: Cloning ofMaytenus emarginata (Willd.) Ding Hou-a tree of the Indian Desert, through tissue culture.—Plant Cell Rep.11: 449–451, 1992.
Sharma, S.: A census of rare and endemic flora of South-East Rajasthan.—In: Jain, S.K., Rao, R.R. (ed.): Threatened Plants of India. Pp. 630–670. Botanical Survey of India, Howrad 1983.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Purohit, S.D., Tak, K. & Kukda, G. In vitro propagation ofBoswellia serrata Roxb. Biol Plant 37, 335–340 (1995). https://doi.org/10.1007/BF02913975
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02913975