Summary
An in vitro method for cloning and mass multiplication of Maytenus emarginata, a highly drought resistant tree of the Indian Desert, has been developed. Shoot segments harvested from a “plus” tree (30-year-old) were cultured to produce multiple shoots (10–15 shoots/explant) on MS medium containing 0.1 mgl−1 IAA and 2.5mgl−1 BAP. In vitro produced shoots were cut into segments and cultured on shoot proliferation medium but with only 1.0 mgl−1 of BAP to further multiply the shoots. Isolated individual shoots were cultured on a filter paper bridge in half strength MS liquid medium containing 25 mgl−1 of IBA for 72 h in the dark at 28±20 C for induction of root(s). About 70–80 percent of shoots rooted. The treelets developed were hardened and transferred to pots. Around 20,000 plants can be obtained from a single explant within a period of 6 months. The protocol is highly reproducible and efficient.
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Abbreviations
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- NAA:
-
α-naphthalene acetic acid
- NOA:
-
β-naphthoxy acetic acid
- BAP:
-
6-benzylaminopurine
- Kn:
-
6-furfurylaminopurine
- B5:
-
Gamborg et al. (1968) medium
- MS:
-
Murashige and Skoog (1962) medium
References
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Communicated by F. Constabel
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Rathore, T.S., Deora, N.S. & Shekhawat, N.S. Cloning of Maytenus emarginata (Willd.) Ding Hou — a tree of the Indian Desert, through tissue culture. Plant Cell Reports 11, 449–451 (1992). https://doi.org/10.1007/BF00232688
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DOI: https://doi.org/10.1007/BF00232688