Abstract
Recombinant eucaryotic expression vector pLXSN/s-bcl-2 has been constructed by cloning humanbcl-2 cDNA containing the full-length open reading frame into the vector pLXSN in sense orientation, and a mammalian cell model expressing human bcl-2 protein has been established by electroporating the recombinant vector into mouse L929 cells. bcl-2 expression in L929 cells has no effect on the cell growth and survival under normal culture conditions, but it can enhance the survival of the cell in the challenge of some apoptosis-inducing stimuli, including tumor necrosis factor α(TNF α) and staurosporine (STS).
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Kamada, S., Shimono, A., Shinto, Y.et al., bcl-2 deficiency in mice leads to pleiotropic ebnormalities: accelerated lymphoid cell death in thymus and spleen, polycystic kidney, hair hypopigmentation, and distorted small intestine,Cancer Res., 1995, 55: 354.
Thompson, C. H., Apoptosis in the pathogenesis and treatment of disease,Science, 1995, 267: 1–456.
Tsujimoto, Y., Croce, C. M., Analysis of the structure, transcripts, and protein products ofbcl-2, the gene involved in human follicular lymphoma,Proc. Natl. Acad. Sci. USA, 1986, 83:5–214.
Zhang, K. Z., Westberg, J. A., Hölttä, E.et al., bcl-2 regulates neural differentiation.Proc. Natl. Acad. Sci. USA, 1996, 93: 4504.
Miyazaki, T., Liu, Z. J., Kawahara,.A.et al., Three distinct IL-2 signaling pathways mediated by bcl-2, c-myc, and Ick cooperate in hernatopoietic cell proliferation,Cell. 1995, 81: 223.
Clien, M, Quintans, J., Fuks, Z.et al., Suppression of bcl-2 messenger RNA production may mediate apoptosis after ionizing radiation, tumor necrosis factor alpha, and ceramide,Cancer Res., 1995, 55: 991.
Genestier, L., Bonnefoy-Berard, N., Rouault, J. P.et al., Tumor necrosis factor-alpha up-regulates bcl-2 expression and decreases calcium-dependent apoptosis in human B cells lines,Int. Immunol., 1995, 7: 533.
Hsu, H., Shu, H. B., Pan, M. G.et al., TRADD-TRAF2 and THADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways.Cell, 1996, 84: 299.
Antwerp, D. J. V., Martin, S. J., Kafri, T.et al., Suppression of TNF-α-induced apoptosis by NF-kB,Science, 1996, 274: 787.
Takayama, S., Sato, T., Krajewski, S.et al., Cloning and functional analysis of RAG-l: a novel Bcl-2-binding protein with anti-cell death activity,Cell, 1995, 80: 279.
Author information
Authors and Affiliations
About this article
Cite this article
He, F., Zhu, X. & Huang, Y. Construction and application of L929 cell model expressing human bcl-2 protein. Chin.Sci.Bull. 44, 1095–1098 (1999). https://doi.org/10.1007/BF02886133
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02886133