Abstract
A medium containing low amounts of nitrogen (19–23 meq.l−1) produced optimum results in micropropagation as revealed by the number of nodes, internode length, chlorophyll content, and leaf area of four potato cvs. belonging each to four different maturity groups. Decreasing amounts of nitrogen also increased chlorophyll content in all four cultivars tested. The NH +4 concentration did not have an effect on micropropagation for low nitrogen supplies.
In all cvs., except Baraka, there was a “carry over” effect of the nitrogen content in the micropropagation medium onto subsequent tuberization, the lower nitrogen (23 meq.l−1) advancing tuber initiation. Microtuberization of cv. Jaerla was earlier in darkness than under short days regardless of the propagation medium used.
Resumen
Un medio con bajo contenido en nitrógeno (19–23 meq.l−1) permitió obtener unos resultados óptimos en la micropropagación que fue evaluada midiendo el número de nudos, la longitud de entrenudos, el contenido en clorofila y el área foliar de 4 cultivares de papa pertenecientes a 4 grupos de madurez distintos. La disminución de nitrógeno en el medio producía un aumento en el contenido en clorofila en los 4 cultivares ensayados. La concentration de NH4+ no modificó la micropropagación para bajos aportes de nitrógeno.
En todos los cultivares, excepto Baraka, hubo un efecto retardado del contenido de nitrógeno en el medio de micropropagación sobre la posterior tuberización, de manera que un contenido bajo de nitrógeno (23 meq.l−1) anticipé la tuberizaeión. La microtuberización del cv. Jaerla fue más precoz en oscuridad que en días cortos, independientemente del medio de propagation utilizado.
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Literature Cited
Amézqueta, J.M., A.M. Mingo-Castel, and E. Tortosa. 1989. Meristematic shoot tip culture and micropropagation in potato (Solanum tuberosum L.) cv. Kennebec and Jaerla. Investigación Agraria. Producrión y Protección Vegetales 4(1):7–17.
Amirouche, L., T. Stuchbury, and S. Matthews. 1985. Comparisons of cultivar perfomance on different nutrient media in a routine method for potato micropropagation. Potato Res 28:469–478.
Caligari, P.D.S. and W. Powell 1989. Variability in response of potato cultivars to micropropagation. I.In vitro performance. Ann Appl Biol 115:115–121.
Dobranszki, J. and M. Mandi 1993. Induction ofin vitro tuberization by short day period and dark treatment of potato shoots grown on hormone-free medium. Acta Biologica Hungarica 44:411–420.
Evans, N.E. 1993. A preliminary study on the effects on nitrogen supply on the growthin vitro of nine potato genotypes (Solanum spp.). J Exp Bot 44:837–841.
Ewing, E.E. and P.C. Struik 1992. Tuber formation in potato: induction, initiation and growth. Hort Rev 14:89–198.
Garner, N. and J. Blake 1989. The induction and development of potato microtubersin vitro on media free of growth regulating substances. Ann Bot 63:663–674.
Krauss, A. 1985. Interaction of nitrogen nutrition, phytohormones and tuberization.In: Potato Physiology. P.H. Li (ed.). Academic Press, Inc., Orlando. pp. 209–230.
Levy, D., A. Seabrook, and S. Coleman 1993. Enhancement of tuberization of axillary shoot buds of potato (Solanum tuberosum L. cultivars culturedin vitro. J Exp Bot 44:381–386.
Mingo-Castel, A.M., A.M. Pelacho, and M.R. de Felipe 1991. Amyloplast division in kinetin induced potato tubers. Plant Sci 73:211–217.
Miller, P.R., L. Amirouche, T. Stuchbury, and S. Matthews 1985. The use of plant growth regulators in micropropagation of slow-growing potato cultivars. Potato Res 28:479–486.
Murashige, T. and F. Skoog 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497.
Pierik, R.L.M. 1987. In vitro culture of higher plants. Martinus Nijhoff Publishers. The Netherlands. p. 344.
Seabrook, J.E.A., S. Coleman, and D. Levy 1993. Effect of photoperiod onin vitro tuberization of potato (Solanum tuberosum L.) Plant Cell Tissue Organ Cult 34:43–51.
Slimmon, T., S. Machado, and R. Coffin 1989. The effect of light onin vitro microtuberization of potato cultivars. Am Potato J 66:843–848.
Teixeira, D.M.C. and J.E.B.P. Pinto 1991. Minituberization of potatoes at different levels of nitrogen, saccharose and BAP. Rev Brasil Fisiol Veg 3:77–83.
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Zarrabeitia, A., Lejarcegui, X., Veramendi, J. et al. Influence of nitrogen supply on micropropagation and subsequent microtuberization of four potato cullwars. American Potato Journal 74, 369–378 (1997). https://doi.org/10.1007/BF02852776
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DOI: https://doi.org/10.1007/BF02852776