Summary
We successfully micropropagatedHesperaloe parviflora from mature plants. Shoot cultures were directly initiated from mature plants using pedicel bud explants on a modified Murashige and Skoog medium containing Nitsch and Nitsch vitamins and 1 μM zeatin riboside. Axillary shoot multiplication from established cultures was most responsive to changing concentrations of N6-benzyladenine (BA) with the greatest production of 6 μM BA. Growing shoots on a medium supplemented with 6 μM BA for 6 wk and then transferring cultures to a 1 μM BA medium for 6 more wk increased the number of transferable shoots, but not significantly. However, our data predicts that the maximum number of transferable shoots produced from a single microshoot would occur on media with 5.4 μM zeatin riboside. Shoots rooted easilyin vitro orex vitro and rooted shoots were easily acclimatized. The methods described in this paper are being used to commercially micropropagateH. parviflora.
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Richwine, A.M., Tipton, J.L. & Thompson, G.A. Micropropagation ofHesperaloe parviflora . In Vitro Cell.Dev.Biol.-Plant 32, 262–266 (1996). https://doi.org/10.1007/BF02822698
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DOI: https://doi.org/10.1007/BF02822698