Abstract
The methylation status of the nuclear DNA from a mealybug, aPlanococcus species, has been studied. Analysis of this DNA by High Performance Liquid Chromatography and Thin Layer Chromatography revealed the presence of significant amounts of 5-—methylcytosine. Since analysis of DNA methylation using the Msp I/Hpa II system showed only minor differences in susceptibility of the DNA to the two enzymes, it seemed possible that 5-methylcytosine (5mC) occurred adjacent to other nucleotides in addition to its usual position, next to guanosine. This was verified by dinucleotide analysis of DNA labelledin vitro by nick translation. These data show that the total amount of 5-methylcytosine in this DNA is slightly over 2.3 mol %, of which 0.61% occurs as the dinucleotide 5mCpG, 0.68% as 5mCpA, 0.59% as 5mCpT and 0.45% as 5mCpC. 5mCpG represents approximately 3.3% of all CpG dinucleotides. The experimental procedure would not have permitted the detection of 5mCp5mC, if it occurs in this system. Unusually high amounts of 6-methyladenine (approximately 4 mol %) and 7-methylguanine (approximately 2 mol %) were also detected, 6-methyladenine and 7-methylguanine occurred adjacent to all four nucleotides. The total G+C content was 33.7% as calculated from dinucleotide data and 32.9% as determined from melting profiles.
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Abbreviations
- 5mC:
-
5-Methylcytosine
- 6mA:
-
6-methyladenine
- 7mG:
-
7-methylguanine
- PLC:
-
high performance liquid chromatography
- TLC:
-
thin layer chromatography
- UV:
-
ultra-violet
- dNTP:
-
deoxynucleotide triphosphates
- DEAE:
-
diethylaminoethyl
- Tris:
-
Tris-(hydroxymethyl) amino methane
References
Banks, H. J. (1976) inResearch Development Bulletin 127, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 51.
Brown, S. W. (1959)Chromosoma,10, 278.
Brown, S.W. and Chandra, H. S. (1977) inCell Biology: A Comprehensive Treatise, eds L. Goldstein and D. M. Prescott (New York: Academic Press) Vol. 1, p. 109.
Brown, S. W. and Nelson-Rees, W. A. (1961)Genetics,46, 983.
Brown, S. W. and Nur, U. (1964)Science,145, 130.
Brown, S. W. and Weigmann, L. I. (1969)Chromosoma,28, 255.
Busch, H. and Daskal, Y. (1977) inMethods in Cell Biology, eds G. Stein, J. Stein and L.J. Kleinsmith (New York: Academic Press) Vol. 16, 1.
Cedar, H., Solage, A., Glasev, G. and Razin, A. (1979)Nucleic Acids Res.,6, 2125.
Chandra, H. S. (1963a)Chromosoma,14, 330.
Chandra, H. S. (1963b)Chromosoma,14, 310.
Chandra, H. S. and Brown, S. W. (1975)Nature (London),253, 165.
Culp, L. A., Dore, E. and Brown, G. M. (1970)Arch. Biochem. Biophy.,136, 73.
Ehrlich, M. and Wang, R. Y-H. (1981)Science,212, 1350.
Gruenbaum, Y., Naveh-Many, T., Cedar, H. and Razin, A. (1981)Nature (London),297, 860.
Holliday, R. andPugh J. E. (1975)Science,187, 226.
Hughes-Schrader, S. (1948)Adv. Genet.,2, 127.
Hughes-Schrader, S. and Ris, H. (1941)j Exp. Zool.,87, 429.
Liskay, R. M. and Evans, R. J. (1980)Proc. Natl. Acad. Sci., (USA),77, 4895.
Loewus, M. W., Brown, S. W. and McLaren, A. D. (1964)Nature (London),203, 104.
Mandel, M. and Marmur, J. (1968)Methods Enzymol,12B, 195.
Mohandas, T., Sparkes, R. S. and Shapiro, L. J. (1981)Science,211, 393.
Nikolskaya, I. I., Lopatina, N. G. and Debov, S. S. (1976)Biochim, Biophys. Acta,435, 206.
Nur, U. (1963)Chromosoma,14, 123.
Nur, U. (1971)Am. Zool.,11, 301.
Rao, G. P. and Chandra, H. S. (1980a) Abstract No. 1.1.5, 7th International Chromosome Conference (Oxford).
Rao, G. P. and Chandra, H. S. (1980b) Abstracts, 2nd International Cell Biology Congress (Berlin),Europ. j Cell. Biol.,22, G30.
Rigby, P. W. J., Dieckmann, M., Rhodes, C. and Berg, P. (1977)j Mol. Biol.,113, 237.
Riggs, A. D. (1975)Cytogenet. Cell Genet.,14, 9.
Sager, R. and Kitchin, R. (1975)Science,189, 426.
Wyatt, G. R. (1961)Ann. Rev. Entomol.,6, 75.
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Deobagkar, D.N., Muralidharan, K., Devare, S.G. et al. The mealybug chromosome system I: Unusual methylated bases and dinucleotides in DNA of aPlanococcus species. J Biosci 4, 513–526 (1982). https://doi.org/10.1007/BF02704646
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DOI: https://doi.org/10.1007/BF02704646