Abstract
A method has been developed for the analysis of isomeric monoglycerides by thin-layer adsorption chromatography. Isomeric monoglycerides are separated on silica gel impregnated with boric acid; a mixed solvent system is used. The interaction of hydroxy compounds with boric acid allows the separation of 1- and 2-monoglycerides which cannot be resolved on silica gel alone. In order to make the method quantitative, the resolved components are charred and their spot densities measured. Glycerol, fatty acids, diglycerides, and triglycerides do not interfere. The method does not induce isomerization.
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Thomas, A.E., Scharoun, J.E. & Ralston, H. Quantitative estimation of isomeric monoglycerides by thin-layer chromatography. J Am Oil Chem Soc 42, 789–792 (1965). https://doi.org/10.1007/BF02631865
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DOI: https://doi.org/10.1007/BF02631865