An approach specific for triacylglycerides (TAGs) is proposed for chromatographic separation of compounds with different chromophores using spectrophotometric detection instead of preliminary calibration of the detector using unavailable (or difficultly accessible) reference standards. A wavelength similar to an isosbestic point (278 nm) is found for this approach for TAGs containing radicals of three isomeric conjugated octadecatrienoic acids (COAs) and can be used to determine the ratio of the amounts of TAGs with isomeric radicals without introducing correction factors for the sensitivity of each compound. A mathematical procedure for processing UV spectra of TAGs containing COA radicals is proposed for finding these points. The specifics of the TAG composition allow the isolation and use of highly labile pure compounds to be avoided. The method is developed using seed oils of red valerian (Centranthus DC.), TAGs of which are formed from two isomeric COAs (α- and β-eleosteric), and pomegranate (Punica granatum L.) with radicals of another pair of COAs (punicic and α-eleostearic) as examples.
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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 85, No. 3, pp. 474–479, May–June, 2018.
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Van Nguyen, A., Deineka, V.I. & Deineka, L.A. Spectrophotometric Determination of Oils with Radicals of Conjugated Octadecatrienoic Acids. J Appl Spectrosc 85, 483–487 (2018). https://doi.org/10.1007/s10812-018-0676-7
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DOI: https://doi.org/10.1007/s10812-018-0676-7