Summary
Somatic embryos of soybean [Glycine max (L.) Merr.] are induced on immature cotyledons explanted onto a medium containing moderately high levels of auxin. Germinability of embryos is related to morphologic normality, and both are reduced by excessive exposure to auxin during the induction process. Shoot meristem development was improved by reducing exposure of cotyledonary explants from 30 to 10 to 14 d on 10 mg/liter α-naphthaleneacetic acid (NAA). A 3-d exposure was sufficient to induce embryos, and embryo frequency was not significantly increased by exposures to NAA for more than 1 wk. Embryo frequency was enhanced, however, by transfer after 9 d to fresh medium containing 10 mg/liter NAA. Germination of morphologically normal embryos was achieved without growth regulators, after maturation for 1 mo. on hormone-free medium and desiccation for 1 wk in a sealed, dry container.
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References
Barwale, U. B.; Kerns, H. R.; Widholm, J. M. Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167:473–481; 1986.
Dahmer, M. L.; Collins, G. B.; Hildebrand, D. F. Accumulation of storage proteins of soybean somatic embryos induced on NAA and 2,4-D. In preparation; 1988.
Gamborg, O. L.; Miller, R. A.; Ojima, K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:150–158; 1968.
Ghazi, T. D.; Cheema, H. V.; Nabors, M. W.: Somatic embryogenesis and plant regeneration from embryogenic callus of soybean,Glycine max L. Plant Cell Rep. 5:452–456; 1986.
Goodman, R. M.; Kiser, J. Biotechnology and its impact on the future developments in soybean production and use. In: Shibles, R. ed. World soybean research conference III: Proceedings. Boulder, CO: Westview Press; 1985.
Gray, D. J. Quiescence in monocotyledonous and dicotyledonous somatic embryos induced by dehydration. HortScience 22:810–814; 1987.
Gray, D. J.; Conger, B. V.; Songstad, D. D. Desiccated quiescent somatic embryos of orchardgrass for use as synthetic seeds. In Vitro 23:29–33; 1987.
Halperin, W.; Wetherall, D. F. Adventive embryony in tissue culture of wild carrot,Dauca carota. Am. J. Bot. 51:274–283; 1964.
Hammatt, N.; Davey, M. R. Somatic embryogenesis and plant regeneration from cultured zygotic embryos of soybean (Glycine max L.). J. Plant Physiol. 128:219–226; 1987.
Hartweck, L. M.; Lazzeri, P. A.; Cui, D., et al. Auxinorientation effects on somatic embryogenesis from immature soybean cotyledons. In Vitro 24:821–828; 1988.
Hildebrand, D. F.; Phillips, G. C.; Collins, G. B. Soybean [Glycine max (L.) Merr.] In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry. Berlin and Heidelburg; Springer-Verlag.
Hsu, E. C.; Obendorf, R. L. Compositional analysis ofin vitro matured soybean seeds. Plant Sci. Lett. 27:129–135; 1982.
Kitto, S. L.; Janick, J. Production of synthetic seeds by encapsulating asexual embryos of carrot. J. Am. Soc. Hort. Sci. 110:277–282; 1985.
Lazzeri, P. A.; Hildebrand, D. F.; Collins, G. B. A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol. Biol. 3:160–167; 1985.
Lazzeri, P. A.; Hildebrand, D. F.; Collins, G. B. Soybean somatic embryogenesis: effects of nutritional, physical and chemical factors. Plant Cell Tissue Organ Cult. 10:209–220; 1987.
Lazzeri, P. A.; Hildebrand, D. F.; Sunega, J., et al. Soybean somatic embryogenesis: interactions between sucrose and auxin. Plant Cell Rep. In press.
Lippman, B.; Lippmann, G. Induction of somatic embryos in cotyledonary tissue of soybean,Glycine max L. Merr. Plant Cell Rep. 3:215–218; 1984.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Ranch, J. P.; Oglesby, L.; Zielinski, A. C. Plant regeneration from embryo-derived tissue cultures of soybeans. In Vitro 21:653–657; 1985.
Ranch, J. P.; Oglesby, L.; Zielinski, A. C. Plant regeneration from tissue culture of soybean by somatic embryogenesis. In: Vasil, I. K., ed. Cell culture and somatic cell genetics of plants. Vol. 3. New York, NY: Academic Press; 1986.
Ranch, J. P.; Oglesby, L.; Zielinski, A. C. Factors affecting the initiation and maintenance of embryogenic soybean tissue. Conf. on Molec. and Cell. Biol. of the Soybean. Ames, IA: Iowa State University; 1986.
Rosenberg, L.; Rinne, R. W. Moisture loss as a prerequisite for seedling growth in soybean seeds (Glycine max L. Merr.). J. Exp. Bot. 37:1663–1674; 1986.
Roth, E. J.; Lark, K. G.; Palmer, R. G. Use of tissue culture as a tool in soybean improvement. In: Shibles, R., ed. World soybean research conference III: Proceedings. Boulder, CO: Westview Press; 1985.
Tetu, T.; Sangwan-Norreel, B.; Sangwan, R. Embryogenesis somatique et regeneration in vitro chez trois varietes precoces de soja. C. R. Acad. Sci. Paris. Series III. 305:613–617; 1987.
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This research was funded by Lubrizol Genetics, Inc., Madison, WI.
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Parrott, W.A., Dryden, G., Vogt, S. et al. Optimization of somatic embryogenesis and embryo germination in soybean. In Vitro Cell Dev Biol 24, 817–820 (1988). https://doi.org/10.1007/BF02623653
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DOI: https://doi.org/10.1007/BF02623653