Summary
In recent years advances have been made in detailing the changes in both collagen and noncollagenous proteins caused by a variety of mutations leading to osteogenesis imperfecta. Much less, however, is known about the mineral phase in the affected bone. In this report, we measured the crystallinity of the apatite in bovine OI bone. Line broadening of the 002 reflection (which estimates changes in the long orc axis of the crystals) and of the 310 reflection (which estimates changes in the thickness of the crystals) both show large decreases (30 and 35% respectively). Transmission electron micrograph measurements indicate that these changes were most probably a result of smaller crystals. No decrease in the ash weight of the bone was observed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Sillence D (1981) Osteogenesis imperfecta: an expanding panorama of variants. Clin Orthop Rel Res 156:11–25
Byers PH, Shapiro JR, Rowe D, David KE (1983) Abnormal α-2 chain in type I collagen from a patient with a form of osteogenesis imperfecta. J Clin Invest 71:689–697
Chu ML, Williams CJ, Pepe G, Hirsch JC, Prockop DJ, Ramirez F (1983) Internal deletion in a collagen gene in a perinatal lethal form of osteogenesis imperfecta. Nature 304:78–80
de Wet WJ, Pihlajaniemi T, Myers J, Kelly TE, Prockop DJ (1983) Synthesis of a shortened pro α1 (I) chain and decreased synthesis of pro α2(I) chains in a pro band and with osteogenesis imperfecta. J Biol Chem 258:7721–7728
Steinmann B, Rao VH, Vogel A, Bruckner P, Gitzelmann R, Byers PH, (1984) Cysteine in the triple-helical domain of one allelic product of the alpha I (I) gene of type I collagen produces a lethal form of osteogenesis imperfecta. J Biol Chem 259:11129–11138
Dickson LA, Pihlajaniemi T, Deak SB, Pope MF, Nicholls AC, Prockop DJ, Myers JC (1984) Nuclease S1 mapping of a homozygous mutation in the carboxyl-propeptide coding region of the pro α2 (I) collagen gene in a patient with osteogenesis imperfecta. Proc Natl Acad Sci USA 81:4524–4528
Minor RR, Pihlajaniemi T, Prockop DJ, Denholm LJ, Wootton JAM (1984) Post-translational over-modification of type I, type III and type V collagen in bovine and human osteogenesis imperfecta. J Cell Biol 99:405a
Takagi Y, Veis A, Sauk JJ (1983) Relation of mineralized defects in collagen matrices to noncollagenous protein components. Clin Orthop Rel Res 176:282–290
Dickson IR, Millar EA, Veis A (1975) Evidence for abnormality of bone matrix proteins in osteogenesis imperfecta. Lancet 2:586–587
Dickson IR, Bagga M, Paterson CR (1983) Variations in the serum concentration and urine excretion of α1HS-glycoprotein, a bone-related protein in normal individuals and in patients with osteogenesis imperfecta. Calcif Tissue Int 35:16–20
Denholm LJ, Cole WG (1983) Heritable bone fragility, joint laxity and dysplastic dentin in Friesian calves: a bovine syndrome of osteogenesis imperfecta. Aust Vet J 60:9–17
Fisher LW, Denholm LJ, Conn KM, Termine JD (1986) Mineralized tissue protein profiles in the Australian form of bovine osteogenesis imperfecta. Calcif Tissue Int 38:16–20
Termine JD, Gehron Robey P, Fisher LW, Shimokawa H, Drum MA, Hawkins GR, Cruz JB, Thompson KG (1984) Osteonectin, bone proteoglycan, and phosphophoryn defects in a form of bovine osteogenesis imperfecta. Proc Natl Acad Sci USA 81:2213–2217
Klug HP, Alexander LE (1962) X-ray diffraction procedures. John Wiley and Sons, New York, pp 491–538
Spurr AR (1969) A low-viscosity resin embedding medium for electron microscopy. J Ultrastruct Res 41:115–132
Shupe JL, Eanes ED, Leone NC (1981) Effect of excessive exposure to sodium fluoride on composition and crystallinity of equine bone tumors. Am J Vet Res 42:1040–1042
Posner AS, Eanes ED, Harper RA, Zipkin I (1963) X-ray diffraction analysis of the effect of fluoride on human bone apatite. Arch Oral Biol 8:549–570
Zipkin I, Eanes ED, Shupe JL (1964) Effect of prolonged exposure to fluoride on the ash, fluoride, citrate, and crystallinity of bovine bone. Am J Vet Res 25:1595–1597
Eanes ED, Zipkin I, Harper RA, Posner AS (1965) Smallangle X-ray diffraction analysis of the effect of fluoride on human bone apatite. Arch Oral Biol 10:161–173
Eanes ED, Termine JD (1983) Calcium in mineralized tissues. In: Spiro TG (ed) Calcium in biology. John Wiley & Sons, New York, pp 201–233
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fisher, L.W., Eanes, E.D., Denholm, L.J. et al. Two bovine models of osteogenesis imperfecta exhibit decreased apatite crystal size. Calcif Tissue Int 40, 282–285 (1987). https://doi.org/10.1007/BF02555262
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02555262