Abstract
Soy lecithin was modified by enzymatic transesterification in a solvent-free system. 1,3-SpecificRhizomucor miehei lipase was found to be efficient in the transesterification with lauric acid and oleic acid, where oleic acid was more incorporated into soy lecithin. Phospholipase A2 incorporated lauric acid hardly at all, but it hydrolyzed lecithin efficiently. The mixture of lipase and phospholipase A2 (1:1, w/w) incorporated lauric acid to the same extent as did 1,3-specific lipase alone at the same total enzyme concentration. The main fatty acids replaced were palmitic and linoleic acids by 1,3-specific lipase and its mixture with phospholipase A2, and linoleic and linolenic acids by phospholipase A2 alone, suggesting an improved oxidative stability of the resulting product. Hydrolysis could not be prevented, but it could be regulated by incubation time and by enzyme dosage. The minimal water content for significant incorporation of lauric acid into lecithin was below 0.5% of the weight of the reaction mixture.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Wren, J.,Nutrition and Food Science 35:10 (1974).
Schneider, M. von,Fat Sci. Technol. 94:524 (1992).
Nieuwenhuyzen, W. van,J. Am. Oil Chem. Soc. 58:886 (1981).
Aura, A.M., P. Forssell, A. Mustranta, T. Suortti and K. Poutanen, Ibid.:887 (1994).
Pedersen, K.B., Patent applicationPCT Int. Appl. WO 9103564 (1991).
Svensson, I., P. Adlercreutz and B. Mattiasson,J. Am. Oil Chem. Soc. 69:986 (1992).
Totani, Y., and S. Hara, Ibid.:848 (1991).
Haas, M.J., D.J. Cichowicz, J. Phillips and R. Moreau, Ibid.:111 (1993).
Heisler, A., C. Rabiller and L. Hublin,Biotechnol. Lett. 13:327 (1991).
Mutua, L.N., and C.C. Akoh,J. Am. Oil Chem. Soc. 70:125 (1993).
Svensson, I., P. Adlercreutz and B. Mattiasson,Appl. Microbiol. Biotechnol. 33:255 (1990).
Bloomer, S., P. Adlercreutz and B. Mattiasson,Biocatalysis 5:145 (1991).
Mustranta, A., P. Forssell, A.-M. Aura, T. Suortti and K. Poutanen, Ibid.:181 (1994).
Mustranta, A., T. Suortti and K. Poutanen,J. Am. Oil Chem. Soc. 71:1415 (1994).
Mustranta, A.,Appl. Microbiol. Biotechnol. 38:61 (1992).
Nyberg, H., inModern Methods of Plant Analysis. New Series Vol. 3, Gas Chromatography/Mass Spectrometry, edited by H.F. Linskens and J.F. Jackson, Springer-Verlag, Berlin-Heidelberg, 1986, pp. 67–99.
Kates, M., inTechniques of Lipidology. Isolation, Analysis and Identification of Lipids, 2nd edn., edited by M. Kates, Elsevier Science Publishers B.V., Amsterdam, 1986, p. 410.
Mudd, J.P., inThe Biochemistry of Plants Vol. 4 Lipids: Structure and Function, edited by P.K. Stumpf, Academic Press, New York, 1980, pp. 249–282.
Forssell, P., R. Kervinen, M. Lappi, P. Linko, T. Suortti and K. Poutanen,J. Am. Oil Chem. Soc. 69:126 (1992).
Author information
Authors and Affiliations
About this article
Cite this article
Aura, AM., Forssell, P., Mustranta, A. et al. Transesterification of soy lecithin by lipase and phospholipase. J Am Oil Chem Soc 72, 1375–1379 (1995). https://doi.org/10.1007/BF02546214
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02546214