Abstract
Previous studies have provided evidence that Mg deficiency affects lipid metabolism. The present experiments were designed to assess whether the hypertriglyceridemia associated with Mg deficiency was related to alterations in post-heparin lipase activity (PHLA). Mg-deficient and control diets were pair-fed to weanling Wistar rats for eight days and plasma lipoproteins were separated into various density classes by sequential preparative ultracentrifugation. Triglycerides were significantly increased in chylomicrons and in the very low density lipoprotein, low density lipoprotein and high density lipoprotein (HDL) fractions. Cholesterol and phospholipid levels were significantly lower in the HDL fraction. PHLA in deficient rat was substantially lower than in control rats. The inverse correlation between plasma triglyceride concentration and PHLA strongly suggests that hypertriglyceridemia is the result of defective lipolysis of plasma triglycerides in Mg-deficient rats. Further examination of the PHLA was carried out by salt-mediated inhibition of lipoprotein lipase (LPL) and by heparin sepharose affinity chromatography and purified rat LPL antiserum. The results indicate that hepatic lipase is significantly decreased in Mg-deficient rats but the low PHLA is due mainly to a decline in LPL. However, total LPL activity, that is, both the intracellular and the extracellular oools of LPL in adipose tissue, heart and diaphragm, were unaffected by Mg deficiency. The results suggest that the decrease of LPL activity in the plasma of Mg-deficient rats may be due to a selective decrease in the heparin-releasable pool of enzyme.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- HDL:
-
high density lipoprotein
- HL:
-
hepatic lipase
- IHD:
-
ischemic heart disease
- LDL:
-
low density lipoprotein
- LPL:
-
lipoprotein lipase
- PHLA:
-
post-heparin lipolytic activity
- VLDL:
-
very low density lipoprotein
References
Rayssiguier, Y., Gueux, E., and Weiser, D. (1981)J. Nutr. 111, 1876–1883.
Rayssiguier, Y., and Gueux, E. (1986)J. Amer. Coll. Nutr. 5, 507–510.
Mahfouz, M.M., and Kummerow, F.A. (1989)Lipids 24, 727–732.
Mahfouz, M.M., Smith, T.L., and Kummerow, F.A. (1989)Biochim. Biophys. Acta 1006, 70–74.
Mahfouz, M.M., Smith, T.L., and Kummerow, F.A. (1989)Biochim. Biophys. Acta 1006, 75–83.
Tongyai, S., Rayssiguier, Y., Motta, C., Gueux, E., Maurois, P., and Heaton, F.W. (1989)Am. J. Physiol. 257 (Cell Physiol. 26), C270-C276.
Rayssiguier, Y., and Gueux, E. (1983)Magnesium 2, 132–138.
Nilsson-Ehle, P. (1980)Ann. Rev. Biochem. 49, 667–693.
Nilsson-Ehle, P., and Schotz, M.C. (1976)J. Lipid Res. 17, 536–541.
Corey, J.E., and Zilversmit, D.B. (1977)J. Lab. Clin. Med. 89, 666–674.
Etienne, J., Noé, L., Rossignol, M., Arnaud, C., Vydelingum, M., and Kissebah, A. (1985)Biochim. Biophys. Acta 834, 95–102.
Buccolo, G., and David, H. (1973)Clin. Chem. 19, 476–482.
Allain, C.C., Poon, L.S., Chan, C.S.G., Richmond, W., and Fu, P.C. (1974)Clin. Chem. 20, 470–475.
Takayama, M., Itoh, S., Nagasaki, T., and Tanimizu, I. (1977)Clin. Chim. Acta 79, 83–98.
Oschry, Y., and Eisenberg, S. (1982)J. Lipid Res. 23, 1099–1106.
Luthringer, C., Rayssiguier, Y., Gueux, E., and Berthelot, A. (1988)Brit. J. Nutrition 59, 243–250.
Rayssiguier, Y., Mazur, A., Cardot, P., and Gueux, E. (1989) inMagnesium in Health and Disease (Itokawa, Y. and Durlarh, J., eds.) pp. 199–207, John Libbey, London.
Ecker, R.H. (1989)N. Engl. J. Med. 320, 1060–1068.
Demant, T., Carlson, L.A., Holmquist, L., Karpe, F., Nilsson-Ehle, P., Packard, C.J., and Shepherd, J. (1988)J. Lipid Res. 29, 1603–1611.
Nilsson-Ehle, P. (1987) inLipoprotein Lipase (Borensztajn, J., ed., pp. 59–77, Evener, Chicago.
Hollenberg, C.H. (1959)Am. J. Physiol. 197, 667–670.
Hollenberg, C.H. (1960)J. Clin. Invest. 39, 1282–1287.
Cryer, A., and Jones, H.M. (1978)Biochem. J. 172, 319–325.
Linder, C., Chernick, S.S., Fleck, T.R., Scow, R.O. (1976)Am. J. Physiol. 231, 860–864.
Durlach, J. (1988) inMagnesium in Clinical Practice (Durlach, J., ed.), pp. 220–221, John Libbey Company Ltd., London, England.
Rasmussen, H.S., Aurup, P., Goldstein, K., McNair, P., Mortensen, P.B., Larsen, O.G., and Lawaelz, H. (1989)Arch. Intern. Med. 149, 1050–1053.
Author information
Authors and Affiliations
About this article
Cite this article
Rayssiguier, Y., Noé, L., Etienne, J. et al. Effect of magnesium deficiency on post-heparin lipase activity and tissue lipoprotein lipase in the rat. Lipids 26, 182–186 (1991). https://doi.org/10.1007/BF02543968
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02543968