Abstract
Chemical characteristics of kernels of the shea tree (Butyrospermum parkii) from Ghana were determined in order to design and evaluate studies on a traditional enzyme-assisted fat extraction of the kernels. The effectiveness of a number of cellular degrading enzymes in assisting the shea fat extraction were also tested by treating meals of the kernels with one or more of these enzymes before extraction and comparing the yield with control extractions. Proximate composition of the kernel on dry-matter basis was: total lipids, 59.04%; crude fat, 54.85; protein, 7.81%; total carbohydrates, 34.77%; ash, 2.57%. Starch content was 7.59%; hemicellulose, 10.84%; cellulose, 5.95%; and pectic substances, 2.93%. Total fiber content was 20.35%. The fat extracted by the Soxhlet method was pale-yellow in color and solid at room temperatures. Its physicochemical characteristics were: melting range, 34–36°C; iodine value, 58.53; saponification value, 180.37; and unsaponifiable matter content, 7.48%. The predominant fatty acids were: palmitic (3.55%), stearic (44.44%), oleic (42.41%), linoleic (5.88%) and linolenic (1.66%) acids. The enzyme-assisted extraction tests showed increases in extraction yield when the shea kernel meals were treated with the enzyme(s) before extraction. An increase of about 20% was realized when a protease and an enzyme with both cellulase and hemicellulase activities were used together. These observations confirmed the fact that the shea kernel is a rich source of fat. They also indicate the possibility of improving shea kernel extraction processes by pretreating the kernel meal with cell structure-degrading enzymes.
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References
Salunkhe D.K., and B.B. Desai,Post-Harvest Biotechnology of Oil Seeds, CRC Press, Inc., Boca Raton, 1986, pp. 204–205.
Ata, J.K.B.A., The Study of the Shea Kernel in Relation to the Traditional Process of Shea Fat, Ph.D. Thesis, University of Ghana, Legon-Accra, 1978.
Codd, L.W., K. Dijkhoff, J.H. Fearson, C.J. van Oss, H.G. Roeberson and E.G. Stanford (eds),Materials and Technology, Vol. VIII, Longman J.H. De Bussy, Amsterdam, 1975, pp. 60–61.
Ruyssen, 1957. Cited by J.K.B.A. Ata, in The Study of Shea Kernal in Relation to the Traditional Process of Shea Fat, Ph.D. Thesis, University of Ghana, Legon-Accra, 1978.
Graille, J., M. Pina and D. Montet,Oleagineux 43:188 (1988).
Ratledge, C., and C.A. Boulton, inComprehensive Biotechnology, Vol. 3, Pergamon Press, New York, 1985, pp. 983–1003.
Fullbrook, P.D.,J. Am. Oil Chem. Soc., 60:428 (1983).
Buenrostro, M., and C.A. Lopez Munguia,Biotechnology Lett. 8:505 (1986).
McGlone, O.C., C.A. Lopez Munguia and J.C. Carter,J. Food Sci. 51:695 (1986).
Sosulski, E., F.W. Sosulski and E. Coxworth,J. Am. Oil Chem. Soc., 65:357 (1988).
Cheah, S.C., M.A. Augustin and L.C-L. Ooi,PORIM Bulletin 20, Palm Oil Research Institute of Malaysia, Kuala Lumpur, 1989.
Sosulski, K., and F.W. Sosulski,J. Am. Oil Chem. Soc. 70:825 (1993).
Smith, D.D., Y.C. Agrawal, B.C. Sarkar and B.P.N. Singh,70:825 (1993)
Official Methods of Analysis of the Association of Official Analystical Chemists, Association of Official Analytical Chemists, Washington, D.C., 1984, Methods 27.005, 27.0066, 27.007, 27.009, 28.014 and 28.024.
Folch, J., M. Lees and G.S.H. Stanley,J. Biol. Chem. 226:497 (1957).
Lee, S.C., L. Prosky and J.W. De Vries,J. Assoc. Off. Anal. Chem. 75:395 (1992).
Pearson, D.,The Chemical Analyses of Foods, 7th edn., Churchill Livingstone, 1976.
Southgate, D.A.T.,J. Sci. Fd. Agric. 20:327 (1969).
Kobayashi, M., K. Funane, H. Ueyama, M. Tanaka and Y. Kato,Biosci. Biotech. Biochem. 57:998 (1993).
Sabir, M.A., F.W. Sosulski and N.W. Hamon,J. Agr. Food Chem. 23:16 (1975).
Southgate, D.A.T.,20:331 (1969).
Dubois, M., K.A. Gills, J.K. Hamilton, P.A. Rebers and F. Smith,Analy. Chem. 28:350 (1950).
Aye, F.O., and D. Adomako,Processing of Shea Fruits, Rep. Cocoa Research Institute of Ghana, 1986/87.
Bailey, A.E.,Industrial Oils and Fats Products. Interscience Publication Incorporated, New York, 1951.
Pesquet, J.-J.,Aproma/Bimonthly Review 27–29:8 (1992).
Lozano, Y.F., C. Dhuique Mayer, C. Bannon and E.M. Gaydou,J. Am. Oil Chem. Soc. 70:561 (1993).
Godin, V.J., and P.C. Spensley;Crop and Product Digest, No. 1, Tropical Products Institute, London, 1971.
Swern D. (ed.)Bailey’s Industrial Oil and Fat Products, Vol. 2, 4th edn., A. Wiley-Interscience Publication, John Wiley & Sons, New York, 1982, pp. 372–376.
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Tano-Debrah, K., Ohta, Y. Enzyme-assisted aqueous extraction of fat from kernels of the shea tree,Butyrospermum parkii . J Am Oil Chem Soc 71, 979–983 (1994). https://doi.org/10.1007/BF02542265
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DOI: https://doi.org/10.1007/BF02542265