Abstract
Functional properties (solubility, foaming capacity and stability, emulsifying capacity, emulsion stability, heat coagulability, heat gelation and film formation) of protein extracted by 45% ethanol/55% 0.1 M NaOH from flaked, defatted, undergermed corn during the Sequential Extraction Process (SEP) were evaluated and compared with those of a laboratory-prepared soy protein concentrate. SEP is a new approach to corn fractionation that recycles the ethanol produced from the fermentation of cornstarch to unstream steps of protein extraction and the simultaneous extraction of corn oil and dehydration of the ethanol. Freeze-dried corn protein extracts contained at least 80% crude protein (dry basis), which is indicative of protein concentrates. SEP protein concentrates had solubilities in water of greater than 80% at pH values of 7 or above and were significantly more soluble than the soy protein concentrate at pH above 3. SEP corn proteins also showed better heat stabilities and greater emulsifying capacities and emulsion stabilities. Dilute dispersions (0.1%) of corn protein produced substantial but less stable foams. Corn proteins produced films similar to zein and soy protein films but were unable to form heat-induced gels. These results indicate that SEP produces a protein concentrate with functional properties suitable for food and industrial uses.
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References
Gnanasambandam, R., and J.F. Zayas,J. Food Sci. 57:829 (1992).
USDA Economic Research Service,Feed Situation and Outlook Yearbook, February 1992.
Wright, K.N., inCorn: Chemistry and Technology, edited by S.A. Watson, and P.E. Ramstad, American Association of Cereal Chemists, Inc., St. Paul, 1987, p. 447.
Hojilla-Evangelista, M.P., D.J. Myers and L.A. Johnson,J. Am. Oil Chem. Soc. 69:199 (1992).
Wilson, C.M., inCorn: Chemistry and Technology, edited by S.A. Watson, and P.E. Ramstad, American Association of Cereal Chemists Inc., St. Paul, 1987, p. 273.
Hojilla-Evangelista, M.P., L.A. Johnson and D.J. Myers,Cereal Chem. 69:643 (1992).
American Society for Testing and Materials Standard Method E 203-75 (Reapproved 1986), American Society for Testing and Materials Standard Method, Philadelphia, 1975.
Approved Methods of the American Association of Cereal Chemists, 8th edn., American Association of Cereal Chemists, St. Paul, 1983.
Robyt, J.F., and B.J. White,Biochemical Methods: Theory and Practice, Waveland Press, Inc., Prospect Heights, 1987, p. 234.
Balmaceda, E.A., M.K. Kim, R. Franzen, B. Mardones and J.C. Lugay, inFood Protein Chemistry, edited by J.M. Regenstein, and C.E. Regenstein, Academic Press, Inc., Orlando, 1984, p. 279.
Hung, J.,Studies on Processing, Functional Characteristics and Nutritional Quality of Hydrothermal Extracts of Soybeans, Ph.D. dissertation, Kansas State University, Manhattan, 1984.
Pearce, K.N., and J.E. Kinsella,J. Agric. Food. Chem. 26:716 (1978).
Fligner, K.L., and M.E. Mangino, inInteractions of Food Proteins, edited by N.P. Parris, and R.B. Barford, American Chemical Society, Washington, D.C., 1991, p. 1.
Zayas, J.F., and C.S. Lin,J. Food Process Preserv. 13:161 (1989).
Hung, S.C., and J.F. Zayas,J. Food Sci. 57:372 (1992).
Phillips, L.G., W. Schulman and J.F. Kinsella,55:1116 (1990).
Kinsella, J.E., inCrit. Rev. Food Sci. and Nutr. 7(3):219 (1976).
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Myers, D.J., Hojilla-Evangelista, M.P. & Johnson, L.A. Functional properties of protein extracted from flaked, defatted, whole corn by ethanol/alkali during sequential extraction processing. J Am Oil Chem Soc 71, 1201–1204 (1994). https://doi.org/10.1007/BF02540537
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DOI: https://doi.org/10.1007/BF02540537