Abstract
The effect of cholesteryl sulfate, a natural membrane component, on the physical state of dipalmitoyl phosphatidylcholine multilamellar vesicles was investigated using fluorescence polarization and differential scanning calorimetry techniques. Cholesteryl sulfate increased the order of acyl chains for those temperatures higher than the gel-to-liquid crystalline transition temperature while it decreased the order for those temperatures below the phase transition temperature. At equimolar concentrations, cholesteryl sulfate suppressed the crystal liquid-to-gel phase transition of dipalmitoyl phosphatidylcholine. These data suggest that sterol sulfates may provide new tools for the elucidation of molecular mechanisms involved in sterollipid interactions.
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Abbreviations
- DPH:
-
1,6-diphenyl-1,3,5-hexatriene
- DPPC:
-
L-α-dipalmitoylphosphatidylcholine
- Chol SO4 :
-
cholesteryl sulfate
- Chol:
-
choleserol
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Le Grimellec, C., Daigneault, A., Bleau, G. et al. Cholesteryl sulfate-phosphatidylcholine interactions. Lipids 19, 474–477 (1984). https://doi.org/10.1007/BF02537413
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DOI: https://doi.org/10.1007/BF02537413