Abstract
Analytical methods to obtain the detailed compositions of the fatty acids in oils containing more than one conjugated octadecatrienoic acid by open-tubular gas liquid chromatography (GLC) and by reversed-phase high performance liquid chromatography (HPLC) were established. Effective GLC separations ofcis,trans,trans-9,11,13-octadecatrienoic acid (ctt-9,11,13–18∶3),ctc-9,11,13–18∶3,ttc-9,11,13–18∶3,ttt-9,11,13–18∶3,ttc-8,10,12–18∶3, andttt-8,10,12–18∶3 were obtained with an opentubular column coated with the nonpolar liquid phase OV-1 using an instrument having all-glass carrier gas pathways. The HPLC method also gave satisfactory separations for the isomeric conjugated octadecatrienoates on the basis of number of thecis andtrans double bonds. Two or three minor conjugated trienoic acids were found along with the principal conjugated trienoic acid in tung oil, and seed oils of cherry,Prunus sp., Momordica charantia, Trichosanthes anguina, Punica granatum, Catalpa ovata, andCalendula officinalis. The mechanism for the formation of the conjugated trienoic acid mixtures in the seed oils is discussed. TheC. ovata seed oil also containedct andtt-9,12-octadecadienoic acids. Thett isomer is presumed to be a precursor ofttc-9,11,13–18∶3, the main conjugated trienoic acid in this oil.
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Hopkins, C.Y., and Chisholm, M.J. (1968) J. Am. Oil Chem. Soc. 45, 176–182.
Smith, C.R., Jr. (1967) in Progress in Chemistry of Fats and Other Lipids (Holman, R.T., ed.) Vol. XI, Part 1, pp. 137–177, Pergamon Press, London.
Hopkins, C.Y. (1972) in Topics in Lipid Chemistry (Gunstone, F.D., ed.) Vol. 3, pp. 37–87, Logos Press, London.
Tulloch, A.P., and Bergter, L. (1979) Lipids 14, 996–1002.
Sonntag, N.O.V. (1979) in Bailey's Industrial Oil and Fat Products (Swern, D., ed.) Vol. 1, p. 34, John Wiley & Sons, New York.
Scholfield, C.R., Butterfield, R.O., Peters, H., Glass, C.A., and Dutton, H.J. (1967) J. Am. Oil Chem. Soc. 44, 50–54.
Tolberg, W.E., and Wheeler, D.H. (1958) J. Am. Oil Chem. Soc. 35, 385–388.
Pitt, G.A.J., and Morton, R.A. (1957) in Progress in Chemistry of Fats and Other Lipids (Holman, R.T., Lundberg, W.O., and Malkin, T., eds.) Vol. IV, pp. 227–278, Pergamon Press, London.
O'Connor, R.T., Heinzelman, D.C., Pack, F.C., and Plank, R.W. (1953) J. Am. Oil Chem. Soc. 30, 182–186.
Tulloch, A.P., and Craig, B.M. (1964) J. Am. Oil Chem. Soc. 41, 322–326.
Wolff, I.A., and Miwa, T.K. (1965) J. Am. Oil Chem. Soc. 42, 208–215.
Conacher, H.B.S., Gunstone, F.D., Hornby, G.M., and Padley, F.B. (1970) Lipids 5, 434–441.
Takagi, T., and Craig, B.M. (1964) J. Am. Oil Chem. Soc. 41, 660–661.
Kleiman, R., Spencer, G.F., and Earle, F.R. (1969) Lipids 4, 118–122.
Toyama, Y., and Takagi, T. (1961) Res. Rept. Nagoya Ind. Sci. Res. Inst. 13, 29–33.
Chisholm, M.J., and Hopkins, C.Y. (1966) J. Am. Oil Chem. Soc. 43, 390–392.
Conacher, H.B.S., and Gunstone, F.D. (1970) Lipids 5, 137–141.
Gunstone, F.D. (1966) Chem. Ind., 1551.
Nelson, M.S., and Pattee, H.E. (1977) Lipids 12, 418–422.
Harlow, R.D., Litchfield, C., and Reiser, R. (1963) J. Am. Oil Chem. Soc. 40, 505–506.
Ackman, R.G., and Hooper, S.N. (1973) J. Chromatogr. 86, 73–81.
Chisholm, M.J., and Hopkins, C.Y. (1963) Can. J. Chem. 41, 1888–1892.
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Takagi, T., Itabashi, Y. Occurrence of mixtures of geometrical isomers of conjugated octadecatrienoic acids in some seed oils: Analysis by open-tubular gas liquid chromatography and high performance liquid chromatography. Lipids 16, 546–551 (1981). https://doi.org/10.1007/BF02535054
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DOI: https://doi.org/10.1007/BF02535054