Abstract
The metabolism of 1-14C-methyl linoleate hydroperoxide (1-14C-MLHP) by the rabbit was investigated. Administration of 1.1–1.9 mg of 1-14C-MLHP by ear vein injection proved lethal to four of the nine experimental animals. After 2 hr the lungs and liver contained 3.3% and 7.2%, respectively, of the dose. This radioactivity was found to be associated primarily with intact 1-14C-MLHP. The triglycerides from these tissues also contained14C-trienoic and14C-dienoic fatty acids. Of the dose, 68% was recovered as14CO2 in 2 hr compared to 39% after 1-14C-methyl linoleate injection. The triglycerides from kidney adipose tissue contained a small amount of14C-hydroxy fatty acid, providing confirmation of previous evidence for the presence of a fatty acid hydroperoxide reductase in animal tissues.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Tappel, A.L., in “Symposium on Foods: Lipids and Their Oxidation,” Edited by H.W. Schultz, E.A. Day and R.O. Sinnhuber, The Avi Publishing Co. Inc., Westport, Connecticut, 1962 p. 122–138.
Tappel, A.L., Fed. Proc. 24:73–84 (1965).
Witting, L.A., Ibid. 24:912–916 (1965).
Bunyan, J., J. Green, E.A. Murrell, A.T. Diplock and M.A. Cawthorne, Brit. J. Nut. 22:97–110 (1968).
Yoshikawa, M., and S. Hirai, J. Gerontol. 22:162–165 (1967).
Witting, L.A., and M.K. Horwitt, Lipids 2:89–96 (1967).
Hendley, D.D., A.S. Mildvan, M.C. Reporter and B.L. Strehler, J. Gerontol. 18:250–259 (1963).
Schornagel, H.E., J. Pathol. Bacteriol. 72:267–272 (1956).
Glavind, J., S. Hartman, J. Clemmesen, K.E. Jensen and H. Dam, Acta Pathol. Microbiol. Scand. 30:1–6 (1952).
Tappel, A.L. Nutr. Today 2:2–7 (1967).
Nishida, T., H. Tsuchiyama, M. Inone and F.A. Kummerow, Proc. Soc Exp. Biol. Med. 105:308–312 (1960).
Kokatnur, M.G., J.G. Bergan and H.H. Draper, Ibid. 123:254–258 (1966).
Horgan, V.J., J.S.L. Philpot, B.W. Porter and D.B. Roodyn, Biochem. J. 67:551–558 (1957).
Olcott, H.S., and A. Dolev, Proc. Soc. Expt Biol. Med. 114:820–822 (1963).
Andrews, J.S., W.H. Wendell, J.F. Mead and R.A. Stein, J. Nutr. 70:199–210 (1960).
Freeman, I.P, and P.J. O’Brien, Biochem. J. 102:9p (1967).
Banks, A., S. Fazakerley, J.N. Keay and J.G.M. Smith, J. Sci. Food Agr. 12:724–728 (1961).
Kokatnur, M.G., J.G. Bergan and H.H. Draper, Anal. Biochem. 12:325–331 (1965).
Wheeler, J., Official and Tentative Methods of the AOCS, 1964, Cd-8-53.
Frankel, E.N., in “Symposium on Foods: Lipids and Their Oxidation,” Edited by H.W. Schultz, E.A. Day and R.O. Sinnhuber, The Avi. Publishing Co. Inc., Westport, Conneticut, 1962, p. 51–78.
Folch, J., I. Ascoli, M. Lees, J.A. Meath and F.N. LeBaron, J. Biol. Chem. 191:833–841 (1951).
Hirsch, J., and E.H. Ahrens, J. Biol. Chem. 233:311–320 (1958).
Sweely, C.C., R. Bentley, M. Makita and W.W. Wells, J. Amer. Chem. Soc. 85:2495 (1963).
Chisholm, M.J., and C.Y. Hopkins, JAOCS 43:390–392 (1966).
Phares, E.F., Arch. Biochem. Biophys. 33:173–178 (1951).
Morris, L.J., Chem. Ind. (London) 1962:1238.
Mattic, M., and D.A. Sutton, Chem. Ind. 1953:666.
Glavind, J., E. Sondergaard and H. Dam, Acta Pharmacol. Toxicol. 18:267–277 (1961).
Reber, R.J., and H.H. Draper, Lipids 5:983–987 (1970).
Bergan, J.G., and H.H. Draper, Lipids 5:976–982 (1970).
O’Brien, P.J., and C. Little, Can. J. Biochem. 47:493–499 (1969).
Christophersen, B.O., Biochim. Biophys. Acta 176:463–470 (1969).
Author information
Authors and Affiliations
About this article
Cite this article
Findlay, G.M., Draper, H.H. & Bergan, J.G. Metabolism of 1-14C-methyl linoleate hydroperoxide in the rabbit. Lipids 5, 970–975 (1970). https://doi.org/10.1007/BF02533199
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02533199