Summary
Preparation of biomembrane lipid based stationary phases has been achieved by recycling 1 mM solutions of the appropriate lipid(soybean lecithin phosphatidylcholine, SLPC; phosphatidylcholine, sphingomyelin, phosphatidylethanolamine or phosphatidylserine) in methanol: water (80∶20 v/v) through reversed-phase (C8) HPLC columns for 18 hours at 0.25 mL min−1. The chromatographic characteristics (retention, peak symmetry and reproducibility and phase stability) have been assessed and compared with two commercially available bonded Immobilized Artificial Membrane (IAM) phases (IAM.PC.MG and IAM.PC.DD) by examination of the retention properties of a range of structurally diverse analytes (n=119). The application of the SLPC phase for prediction of analyte lipophilicity (logPoctanol/water) is shown to be comparable to the IAM.PC.MG and superior to the IAM.PC.DD bonded phases. Cross-phase comparison of analyte retention characteristics on four different lipid phases indicate that such phases may provide a rapid evaluation of analyte-lipid interactions. The dynamic coating methodology is economically viable for the small laboratory, rapid and reproducible, resulting in phase surfaces which are stable over longer periods of time than those of the commercially available bonded phases.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Lien, E.J.Ann. Rev. Pharmacol. Toxicol. 1981,21, 31–61.
Barton, P.; Davis, A.M.; McCarthy, D.J.; Webborn, P.J.H.J. Pharm. Sci. 1997,86, 1034–1039.
Hersey, A.; Hill, A.P.; Hyde, R.M.; Livingstone, D.J.Quant. Struct.-Act. Relat. 1989,8, 288–296.
Leo, A.; Hansch, C.; Elkins, D.Chem. Rev. 1971,71, 525–616.
Nelson Smith, R.; Hansch, C.; Ames, M.M.J. Pharm. Sci 1975,64, 599–607.
Ramsden, J.J.Experientia. 1993,49, 688–692.
Tute, M.S.Chemistry and Industry. 1975,1, 100–105.
Dearden, J.C.; Bresnen, G.M.Quant. Struct.-Act. Relat. 1988,7, 133–144.
Mirrlees, M.M.; Moulton, S.J.; Murphy, C.T.; Taylor, P.J.J. Med. Chem. 1976,19, 615–618.
Miyake, K.; Terada, H.J. Chromatogr. 1978,157, 386–390.
Unger, S.H.; Feuerman, T.F.J. Chromatogr. 1979,176, 426–429.
Miyake, K.J.Chromatogr. 1982,240, 9–20.
Garst, J.E.; Wilson, W.C.J. Pharm. Sci. 1984,73, 1616–1623.
Gaspari, F.; Bonati, M.J. Pharm. Pharmacol. 1987,39, 252–260.
Hafkenscheid, T.L.; Tomlinson, E.Int. J. Pharm. 1983,16, 225–239.
Hong, H.; Wang, L.; Zou, G.J. Liq. Chrom. Rel. Tech. 1997,20, 3029–3037.
Konemann, R.; Zelle, R.; Busser, F.; Hammers, W.E.J. Chromatogr. 1979,178, 559–565.
Valko, K.J. Liq. Chromatogr. 1984,7, 1405–1424.
De Biasi, V.; Lough, W.J.; Evans, M.B.J. Chromatogr. 1986,353, 279–284.
Hansen, S.H.J. Chromatogr. 1986,386, 39–47.
Thus, J.L.G.; Kraak, J.C.J. Chromatogr. 1985,320, 271–279.
Ong, S.; Liu, H.; Qiu, X.; Bhat, G.; Pidgeon, C.Anal. Chem. 1995,67, 755–762.
Miyake, K.; Kitaura, F.; Mizuno, N.; Terada, H.J. Chromatogr. 1987,389, 47–56.
Pidgeon, C.; Venkataram, U.V.Anal. Biochem. 1989,176, 36–47.
Ong, S.; Cai, S.J.; Bernal, C.; Rhee, D.; Qiu, X.; Pidgeon, C.Anal. Chem. 1994,66, 782–792.
Yang, C.Y.; Cai, S.J.; Liu, H.; Pidgeon, C.Adv. Drug. Del. Rev. 1996,23, 229–256.
Markovich, R.J.; Qiu, X.; Nichols, D.E.; Pidgeon, C.Anal. Chem. 1991,63, 1851–1860.
Hanna, M.; De Biasi, V.; Bond, B.; Salter, C.; Hutt, A.J.; Camilleri, P.Anal. Chem. 1998,70, 2092–2099.
Craig, P.N.; In Drayton, C.I.; Hansch, C.; Sammes, P.G.; Taylor, J.B. Eds.Cumulative Subject Index and Drug Compendium; Comprehensive Medicinal Chemistry, Volume 6, Pergamon Press, Oxford,1990, 237–291.
Cook, R.D.Technometrics. 1977,19, 15–18.
Cook, R.D.J. Am. Stat. Assoc. 1979,74, 169–174.
Kaliszan, R.; Kaliszan, A.; Wainer, I.W.J. Pharm. Biomed. Anal. 1993,11, 505–511.
Montanari, M.L.C.; Montanari, C.A.; Veloso, D.P.; Cass, Q.B.J. Liq. Chrom. Rel. Tech. 1997,20, 1703–1715.
Valko, K.; Slegel, P.J. Chromatogr. 1993,631, 49–61.
Salminen, T.; Pulli, A.; Taskinen, J.J. Pharm. Biomed. Anal. 1997,15, 469–477.
Austin, R.P.; Davis, A.M.; Manners, C.N.J. Pharm. Sci. 1995,84, 1180–1183.
Barbato, F.; Rotonda, M.I.L.; Quaglia, F.J. Pharm. Sci. 1997,86, 225–229.
Hollinshead C.M.; Hanna, M.; Barlow, D.J.; De Biasi, V.; Bucknall, D.G.; Camilleri, P.; Hutt, A.J.; Lawrence, M.J.; Lu, J.; Su, T.J.J. Pharm. Pharmacol. (Suppl)1999,51, 90.
Krause, E.; Dathe, M.; Weprecht, T.; Bienert, M.J. Chromatogr. A 1999,849, 125–133.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hanna, M., de Biasi, V., Bond, B. et al. Biomembrane lipids as components of chromatographic phases: Comparative chromatography on coated and bonded phases. Chromatographia 52, 710–720 (2000). https://doi.org/10.1007/BF02490994
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02490994