Summary
A simple, low-cost, sensitive and selective HPLC method was developed for the determination of phenazopyridine in human plasma. The method employs UV detection of phenazopyridine and of the internal Standard at 2 different wavelengths. Calibration curves were linear over a large dynamic range, i.e., within 0.05–10.0 μg mL−1 with limit of quantification of 0.05 μg mL−1, and a limit of detection of 0.01 μg mL−1.
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Farin, D., Piva, G. & Kitzes-Cohen, R. Determination of phenazopyridine in human plasma by high performance liquid chromatography. Chromatographia 52, 179–180 (2000). https://doi.org/10.1007/BF02490452
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DOI: https://doi.org/10.1007/BF02490452