Abstract
Protoplasts were isolated enzymatically from prothallia ofLygodium japonicum. The protoplasts grown in a culture medium containing 0.6 M mannitol and 0.05 M sucrose began to divide within 8 days of culture, and after 30 days 10-cell clusters were present. When the cell-clusters were transferred into fresh media followed by sequential reduction of mannitol concentration, they developed rhizoids and protonemata. The reduction of mannitol concentration to 0.3 M resulted in the regeneration of a common gametophyte within 50 days of culture, and subsequently the regenerated gametophytes produced sporophytic leaves and roots.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Albaum, H.G. 1938. Normal growth, regeneration, and adventitious outgrowth formation in fern prothallia. Amer. J. Bot.25: 37–44.
Cocking, E.C. 1972. Plant cell protoplasts-Isolation and development. Ann. Rev. Plant Physiol.23: 29–50.
Evans, P.K. andE.C. Cocking. 1977. Isolated plant protoplasts.In: H.E. Street, ed., Plant Tissue Culture and Cell Culture p. 103–135. University of California Press, Berkeley.
Gay, L. 1976. The development of leafy gametophytes from isolated protoplasts ofPolytrichum junipericum. Z. Pflanzenphysiol.79: 32–39.
Ito, M. 1962. Studies on the differentiation of fern gametophytes I. Regeneration of single cells isolated from cordate gametophytes ofPteris vittata. Bot. Mag. Tokyo75: 19–27.
Kato, Y. 1964. Physiological and morphogenetic studies of fern gametophytes in aseptic culture II. One- and two-dimensional growth in sugar media. Bot. Gaz.125: 33–37.
Maeda, M. 1979. Isolation and culture of protoplasts from moss protonemata. Bot. Mag. Tokyo92: 105–110.
Murashige, T. andF. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15: 473–497.
Partanen, C.R., J.B. Power andE.C. Cocking. 1980. Isolation and division of protoplasts ofPteridium aquilinum. Plant Sci. Lett.17: 333–338.
Schieder, O. andG. Wenzel, 1972. Enzymatic isolation of protoplasts from the liverwortSphaerocarpus donnellii. Aust. Z. Natruforschung27b: 479–480.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Maeda, M., Ito, M. Isolation of protoplasts from fern prothallia and their regeneration to gametophytes. Bot Mag Tokyo 94, 35–40 (1981). https://doi.org/10.1007/BF02490201
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02490201