Summary
Recovery of cellulases from solid state cultures ofTrichoderma harzianum was efficiently achieved by hydraulic pressing. Pressing of fermented solids yielded carboxymethyl-cellulase (CMCase) extraction efficiency of 71% and a ratio of leachate to fermented solids of 0.58 (v/w). Addition of water to pressed solids and second pressing improved the efficiency (95%) with simultaneous increase in the ratio to 1.16 (v/w). The overall extraction of filter paper activity was lower (85%) than that of CMCase. This technique is simple and its extraction efficiency is similar to that obtained in multiple-contact counter-current systems. The hydraulic press in its individuality was not used earlier to leach the product from fermented solids.
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Roussos, S., Raimbault, M., Saucedo-Castañeda, G. et al. Efficient leaching of cellulases produced byTrichoderma harzianum in solid state fermentation. Biotechnol Tech 6, 429–432 (1992). https://doi.org/10.1007/BF02447483
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DOI: https://doi.org/10.1007/BF02447483