Abstract
Astrocyte hypertrophy, associated with neuronal degeneration, has been reported in aging rat hippocampus. Dietary restriction throughout life (restriction of calories without decrease in essential nutrients) increases average and maximal lifespans in rodents. In the present study, the effect of caloric restriction on age-related changes in the maximal diameters of mouse astrocytes was measured. Specific pathogen free C57BL6 mice maintained on either ad libitum (NIH 31 rodent diet) or restricted (60% ad libitum with vitamin supplementation) diets, ages 0.6 (weaning), 6, 19, and 24 months were obtained from the NCTR aging rodent facility in Jefferson, AK. Their brains were stained by a modified Golgi method and astrocyte diameters were determined with a Zeiss Videoplan image analyzer. Astrocytes in the hippocampus and dentate gyrus of ad libitum-fed mice were of similar diameter from 6 to 24 months of age, and were larger than those of weanlings. However, astrocytes were significantly smaller in the hippocampus and dentate gyrus of caloric restricted mice at 19 and 24 months, suggesting the prevention or reversal of age-related astroglial enlargement. Astroglia were also significantly smaller in the frontal and parietal cortices of caloric-restricted mice at six months of age than those of ad libitum-fed mice. At older ages, however, no significant differences were seen between the two groups. This finding may indicate a delay in neocortical astrocyte development by caloric restriction.
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Yu, B.P., Masoro, E.J., Morata, I., Bertand, H.A., and Lynd, F.T.: Life span study of SPF Fischer 344 male rats fed ad libitum or restricted diets: Longevity, growth, lean body mass and disease. J. Gerontol., 37: 130–141, 1982.
Sacher, G.A.: Life table modification and life prolongation, in Handbook of the Biology of Aging, edited by Fine, C.E. and Hayflick, L., New York, Van Nostrand Reinhold, 1977.
Landfield, P.W., Rose, G., Sandles, L., Wohlstadter, T.C., and Zynch, G.: Patterns of astroglial hypertrophy and neuronal degeneration in the hippocampus of aged, memory-deficient rats. J. Gerontol., 32: 3–12, 1977.
Geinisman, Y., Bondareff, W., and Dodge, J.T.: Hypertrophy of astroglial processes in the dentate gyrus of the senescent rat. Am. J. Anatomy, 153: 537–544, 1978.
Klatzo, I.: A study of glia by the golgi method. Lab. Invest., 1: 345–350, 1952.
Silberberg, M., and Silberberg, R.: Diet and life span. Physiol. Rev., 35: 347–359, 1955.
Castiglioni, A.J., Peterson, S.L., Sanabria, E.L., and Tiffany-Castiglioni, E.: Structural changes in astrocytes induced by seizures in a temporal model of epilepsy. J. Neurosci. Res., 26: 334–341, 1990.
Bjorklund, H., Eriksdotter-Nilsson, M., Dahl, D., Rose, G., Hoffer, B., and Olson, L.: Image analysis of GFA-positive astrocytes from adolescence to senescence. Exp. Brain Res., 58: 163–170, 1985.
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Castiglioni, A.J., Legare, M.E., Busbee, D.L. et al. Morphological changes in astrocytes of aging mice fed normal or caloric restricted diets. AGE 14, 102–106 (1991). https://doi.org/10.1007/BF02435015
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DOI: https://doi.org/10.1007/BF02435015