Summary
The uptake and distribution of15NH +4 ,15NO −3 and15N2 was studied in greenhouse-grown beans (Phaseolus vulgaris L.) with a commercial cultivar and 2 recombinant inbred backcross lines;15N was supplied in the nutrient solution at the R3 (50% bloom) stage. Plants were harvested 1, 5 and 10 days after treatment, and were separated into nodules, roots, stems, mature leaflets, immature leaflets, and flowers/fruits. All 3 lines showed rapid increases in the N content of flowers/fruits after the R3 stage. However, the percentage N in these tissues decreased after the R3 stage.
One of the recombinant lines showed a greater uptake of NH +4 than the other 2 lines. Rates of15N2 fixation and NO −3 uptake were similar for all 3 lines, N2 fixation estimated from total N content showed the 2 recombinant lines with 24 and 34 percent greater activity than the commercial cultivar.
Distribution of15N at the whole plant level was similar for all 3 lines for a similar N source.15NO −3 was transported first to leaflets and the label then moved into flowers/fruits. Transport of fixed N2 was from the nodules to roots, stems and into flowers/fruits; usually less than 10 percent entered the leaflets. This indicates that N2 fixation furnishes N directly to flowers/fruits with over 50 percent of the fixed N being deposited into flowers/fruits within 5 days after treatment.
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Dubois, J.D., Burris, R.H. Comparative study of N uptake and distribution in three lines of common bean (Phaseolus vulgaris L.) at early pod filling stage. Plant Soil 93, 79–86 (1986). https://doi.org/10.1007/BF02377147
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DOI: https://doi.org/10.1007/BF02377147