Summary
Short-term absorption experiments were conducted with intact barley (Hordeum vulgare L.) seedlings to observe the effects of the osmotic potential (Ψπ) and salt species on nitrate uptake andin vivo nitrate reduction. The experiments consisted of growing barley seedlings for 5 days in complete nutrient solutions salinized to (Ψπ) levels of −0.6, −1.8, −3.0, −4.2, and −5.4 bars with NaCl, CaCl2 or Na2SO4. After the absorption period, the seedlings were separated into shoots and roots, weighed, then analyzed for NO3. The nutrient solutions were sampled for NO3 analysis each day immediately before renewing the solutions. The accumulative loss of NO3 from the solutions was considered to be uptake whereas NO3 reduction was the difference between uptake and seedling content. Lowering the (Ψπ) of the nutrient solutions resulted in decreased concentrations of NO3 in the plant, little or no effect (except at the lowest (Ψπ) level) on uptake, and increased nitrate reductase activity. Increased rates of NO3 reduction were in particular associated with the Cl concentration of the nutrient solution.
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References
Adriano, D. C., Pratt, P. F. and Holtzclaw, K. M. 1973 Comparison of two simple methods of chlorine analysis in plant materials. Agron. J.65, 133–134.
Ben-Zioni, A. Vaadia, Y. and Lips, S. H. 1971 Nitrate uptake by roots as regulated by nitrate reduction products of the shoot. Physiol. Plant.24, 288–290.
Cataldo, D. A., Haroon, M., Schrader, L. E. and Youngs, V. L. 1975 Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commun. Soil Sci. Plant Anal.6, 71–80.
Cerda, A., Bingham, F. T. and Hoffman, G. J. 1977 Interactive effect of salinity and phosphorus on sesame. Soil Sci. Am. J.41, 915–918.
Cerda, A. and Bingham, F. T. 1978 Yield, mineral composition, and salt tolerance of tomato and wheat as affected by NaCl and P nutrition. Agrochim.XXII, 140–149.
Chantarotwong, W., Huffaker, R. C., Miller, B. L. and Granstedt, R. C. 1976In vivo nitrate reduction in relation to nitrate uptake, nitrate content, andin vitro nitrate reductase activity in intact barley seedlings. Plant Physiol.57, 519–522.
Helal, H. M. and Mengel, K. 1979 Nitrogen metabolism of young barley plants as affected by NaCl-salinity and potassium. Plant and Soil51, 457–462.
Huffaker, R. C., Radin, T., Kleinkopf, G. E. and Cox, E. L. 1970 Effects of mild water stress-on enzymes of nitrate assimilation and of the carboxylative phase of photosynthesis in barley. Crop Sci.10, 471–474.
Langdale, G. W. and Thomas, J. R. 1971 Soil salinity effects on absorption of nitrogen, phosphorus and protein synthesis by coastal bermudagrass. Agron. J.63, 708–711.
Langdale, G. W., Thomas, J. R. and Littleton, T. G. 1973 Nitrogen metabolism of stargrass as affected by nitrogen and soil salinity. Agron. J.65, 468–470.
Mattas, R. E. and Pauli, A. W. 1965 Trends in nitrate reduction and nitrogen fractions in young corn (Zea mays L.) plants during heat and moisture stress. Crop Sci.5, 181–184.
Murarka, I. P., Jackson, T. L. and Moore, D. P. 1973 Effects of N, K, and Cl on nitrogen components of Russet Burbank potato plants (Solanum tuberosum L.). Agron. J.65, 868–870.
Shanner, D. L. and Boyer, J. S. 1976 Nitrate reductase activity in maize (Zea mays L.) leaves. II. Regulation by nitrate flux at low leaf water potential. Plant Physiol.58, 505–509.
Torres, C. and Bingham, F. T. 1973 Salt tolerance of Mexican wheat: I. Effect of NO3 and NaCl on mineral nutrition, growth and grain production of four wheats. Soil Sci. Soc. Am. Proc.37, 711–715.
Torres Bernal, C., Bingham, F. T. and Oertli, J. 1974 Salt tolerance of Mexican wheat: II. Relation to variable sodium chloride and length of growing season. Soil Sci. Soc. Am. Proc.38, 777–780.
Van Kirk, C. A. and Raschke, K. 1978 Presence of chloride reduces malate production in epidermis during stomatal opening. Plant Physiol.61, 361–364.
Yousif, Y. H., Bingham, F. T. and Yermanos, D. M. 1972 Growth, mineral composition, and seed oil of sesame (Sesamum indicum L.) as affected by NaCl. Soil Sci. Soc. Am. Proc.36, 450–453.
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Luque, A.A., Bingham, F.T. The effect of the osmotic potential and specific ion concentration of the nutrient solution on the uptake and reduction of nitrate by barley seedlings. Plant Soil 63, 227–237 (1981). https://doi.org/10.1007/BF02374601
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DOI: https://doi.org/10.1007/BF02374601