Summary
A method is described for assessing the activities of growth substances using excised potato buds. Plugs containing an apical bud were excised and the cut surfaces wrapped in vitafilm. The buds were held for up to thirty days at 20°C without rotting. Daily treatment of buds for 5 days after excision, with either gibberellic acid or an extract of potato sprout promoted breaking of dormancy and growth of sprouts.
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References
Burton, W. G., 1956. Some observations on the growth substances in ether of the potato tuber.Physiologia Pl. 9:567–587.
Es, A. van & K. J. Hartmans, 1969. The influence of abscisin II and gibberellic acid on the sprouting of excised potato buds.Eur. Potato J. 12:59–63.
Goodwin, P. B., 1966. An improved medium for the rapid growth of isolated potato buds.J. exp. Bot. 17:590–595.
Marth, P. C. & E. S. Schultz, 1968. Methods of studying plant hormones and growth-regulating substances. Growth retardation. Vegetative sprout method. Agricultural Handbook 336, U.S. Dept. Agriculture, pp. 68–70.
Rappaport, L., S. Blumenthal-Goldschmidt, M. D. Clegg, & O. E. Smith, 1965. Regulation of bud rest in tubers of potatoSolanum tuberosum L. I. Effect of growth substances on excised potato buds.Pl. Cell Physiol. 6:587–599.
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Harkett, P.J. A potato bud bioassay for use in assessing activities of endogenous growth substances in the potato. Potato Res 19, 381–385 (1976). https://doi.org/10.1007/BF02359857
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DOI: https://doi.org/10.1007/BF02359857