Abstract
A technique is presented for the 3D visualisation of the coronary arterial tree using an imaging cryomicrotome. After the coronary circulation of the excised heart was filled with a fluorescent plastic, the heart was frozen and mounted in the cryomicrotome. The heart was then sliced serially, with a slice thickness of 40μm, and digital images were taken from each cutting plane of the remaining bulk material using appropriate excitation and emission filters. Using maximum intensity projections over a series of images in the cutting plane and perpendicular plane, the structural organisation of intramural vessels was visualised in the present study. The branching end in the smallest visible vessels, which define tissue areas that are well delineated from each other by 1–2 mm wide bands populated only by vessels less than 40 μm in diameter. The technique presented here allows further quantification in the future of the 3D structure of the coronary arterial tree by image analysis techniques.
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References
Bassingthwaighte, J. B., Yipintsoi, T., andHarvey, R. B. (1974): ‘Microvasculature of the dog left ventricular myocardium’,Microvasc. Res.,7, pp. 229–249
Bernard, S. L., Ewen, J. R., Barlow, C. H., Kelly, J. J., McKinney, S., Frazer, D. A., andGlenny, R. W. (2000): ‘High spatial resolution measurements of organ blood flow in small laboratory animals’,Am. J. Physiol. Heart Circ. Physiol.,279, pp. H2043-H2052
Cornelissen, A. J., Dankelman, J., VanBavel, E., Stassen, H. G., andSpaan, J. A. (2000): ‘Myogenic reactivity and resistance distribution in the coronary arterial tree: a model study’,Am. J. Physiol. Heart Circ. Physiol.,278, pp. H1490-H1499
Cornelissen, A. J., Dankelman, J., VanBavel, E., andSpaan, J. A. (2002): ‘Balance between myogenic, flow-dependent, and metabolic flow control in coronary arterial tree: a model study’,Am. J. Physiol. Heart Circ. Physiol.,282, pp. H2224-H2237
Eng, C., Cho, S., Factor, S. M., Sonnenblick, E. M., andKirk, E. S. (1984): ‘Myocardial micronecrosis produced by microsphere embolization. Role of α-adrenergic tonic influence on the coronary microcirculation’,Circulation,54, pp. 74–82
Ince, C., Ashruf, J. F., Avontuur, J. A., Wieringa, P. A., Spaan, J. A., andBruining, H. A. (1993): ‘Heterogeneity of the hypoxic state in rat heart is determined at capillary level’,Am. J. Physiol.,264, pp. H294-H301
Kassab, G. S., Rider, C. A., Tang, N. J., andFung, Y. C. (1993): ‘Morphometry of pig coronary arterial trees’,Am. J. Physiol.,265, pp. H350-H365
Mohlenkamp, S., Lerman, L. O., Bajzer, Z., Lund, P. E., andRitman, E. L. (2002): ‘Quantification of myocardial microcirculatory function with X-ray CT 6350’,Ann. N. Y. Acad. Sci.,972, pp. 307–316
Okun, E. M., Factor, S. M., andKirk, E. S. (1979): ‘End-capillary loops in the heart: an explanation for discrete myocardial infarctions without border zones’,Science,206, pp. 565–567
Palagyi, K., Tschirren, J., andSonka, M. (2003): ‘Quantitative analysis of intrathoracic airway trees: methods and validation’,Inf. Process Med. Imag.,18, pp. 222–233
Streekstra, G. J. andvan Pelt, J. (2002): ‘Analysis of tubular structures in three-dimensional confocal images’,Network.,13, pp. 381–395
Toyota, E., Fujimoto, K., Ogasawara, Y., Kajita, T., Shigeto, F., Matsumoto, T., Goto, M., andKajiya, F. (2002): ‘Dynamic changes in three-dimensional architecture and vascular volume of transmural coronary microvasculature between diastolic- and systolic-arrested rat hearts’,Circulation 105, pp. 621–626
VanBavel E., andSpaan, J. A. E. (1992): ‘Branching patterns in the porcine coronary arterial tree. Estimation of flow heterogeneity’,Circ. Res.,71, pp. 1200–1212
Van Herk, M., de Jaeger, K., de Munck, J., Hoogeman, M., andLebesque, J. ‘A delineation system for N modalities: Software aspects’, 13th ICCR Proc.
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Spaan, J.A.E., ter Wee, R., van Teeffelen, J.W.G.E. et al. Visualisation of intramural coronary vasculature by an imaging cryomicrotome suggests compartmentalisation of myocardial perfusion areas. Med. Biol. Eng. Comput. 43, 431–435 (2005). https://doi.org/10.1007/BF02344722
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DOI: https://doi.org/10.1007/BF02344722