Abstract
Three X chromosome-specific bovine cosmids were used for fluorescencein situ hybridization mapping on reindeer (Rangifer tarandus) chromosomes, to test whether such large genomic clones could be used for comparative mapping across distantly related species. All three cosmids showed distinct unique hybridization sites on the reindeer X. Comparative map locations of these cosmids, together with the relative C-banding and genome size data on the X chromosomes of the two species, provide preliminary indications that the short and long arms of bovine X correspond, respectively, to the long and short arms of the reindeer X. The study also demonstrates that cosmid clones can be used successfully for comparative mapping across species that diverged 35 million years ago.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Chowdhary BP, de la Sena C, Harbitz I, Eriksson I, Gustavsson I (1995) FISH on metaphase and interphase chromosomes demonstrates the physical order of the genes for GPI, CRC and LIPE in pigs.Cytogenet Cell Genet 71: 175–178.
Colbert EH (1969)Evolution of the Vertebrates: a History of the Backboned Animals through Time. New York: John Wiley & Sons.
Fraccaro M, Gustavsson I, Hulten Maj, Lindsten J, Tiepolo L (1968) Chronology of DNA replication in the sex chromosomes of the reindeer (Rangifer tarandus L).Cytogenetics 7: 96–211.
Gripenberg U (1984) Characterization of the karyotype of the reindeer (Rangifer tarandus); the distribution of the heterochromatin in the Reindeer and the Scandinavian moose (Alces alces).Proc of the 6th Eur Colloq Cytogenet Domest Anim 1984, pp 68–79.
Gripenberg U, Wessman M (1993) Restriction endonuclease staining profiles in the C-heterochromatin of Cervidae. II. The sex chromosomes.Hereditas 118: 251–257.
Makino S (1944) A contribution to the study of the chromosomes of domestic mammals.Zool Mag (Tokyo)56: 8–15.
Matthey R (1945) L'évolution de formule chromosomiale chez les vertébrés.Experientia 1: 50–56, 78–86.
Lin CC, Newton DR, Church RB (1977) Identification and nomenclature for G-banded bovine chromosomes.Can J Genet Cytol 19: 271–282.
Nes N, Armud J, Tondevold OB (1965) Chromosome studies in reindeer (Rangifer tarandus).Nord Vet Med 17: 589–593.
Ohno S (1967)Sex Chromosomes and Sex Linked Genes. Berlin: Springer.
Ohno S (1969) The mammalian genome in evolution and conservation of the original X-linkage group, in Benirshke K, ed.Comparative Mammalian Cytogenetics. New York: Springer.
Ohno S, Becak W, Becak ML (1964) X-autosome ratio and the behaviour pattern of individual X chromosomes in placental mammals.Chrosoma (Berlin)15: 14–30.
Pathak S, Stock AD (1974) The X chromosomes of mammals: karyological homology as revealed by banding techniques.Genetics 78: 703–714.
Popescu CP (1969) Ideogram of yak (Bos grunniens), cattle (Bos taurus) and their hybrids.Ann Genet Sel Anim 1: 207–217.
Seabright M (1971) A rapidly banding technique for human chromosomes.Lancet 2: 971–972.
Scherthan H, Cremer T, Arnason U et al. (1994) Comparative chromosome painting discloses homologous segments in distantly related mammals.Nature Genet 6: 342–347.
Author information
Authors and Affiliations
Corresponding author
Additional information
accepted for publication by M. Schmid
Rights and permissions
About this article
Cite this article
Prakash, B., Kuosku, V., Olsaker, I. et al. Comparative FISH mapping of bovine cosmids to reindeer chromosomes demonstrates conservation of the X-chromosome. Chromosome Res 4, 214–217 (1996). https://doi.org/10.1007/BF02254962
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02254962