Summary
Interleukin-1 (IL-1) stimulates fibroblast-mediated hyaluronate (HA) synthesis in vitro. In the present study the degree of polymerization of such HA was studied using HPLC (high performance liquid chromatography) with a size exclusion column combined with125I-HABP assay used to measure the HA concentration in various HA molecular weight fractions separated using HPLC. IL-1 stimulated HA was more polydisperse than that produced by resting fibroblasts with a molecular weight varying from more than 4×106 daltons to less than 7.1×103 daltons. This IL-1 effect may contribute to the low molecular weight HA produced by freshly explanted arthritic synovial tissue and to the low viscosity of arthritic synovial fluid in vivo.
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Konttinen, Y.T., Saari, H. & Nordström, D.C. Effect of interleukin-1 on hyaluronate synthesis by synovial fibroblastic cells. Clin Rheumatol 10, 151–154 (1991). https://doi.org/10.1007/BF02207654
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DOI: https://doi.org/10.1007/BF02207654