Abstract
During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG-6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA and TSG-6 activity in tissues.
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Lauer, M.E., Loftis, J., de la Motte, C., Hascall, V.C. (2015). Analysis of the Heavy-Chain Modification and TSG-6 Activity in Pathological Hyaluronan Matrices. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_42
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DOI: https://doi.org/10.1007/978-1-4939-1714-3_42
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1713-6
Online ISBN: 978-1-4939-1714-3
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