Abstract
In order to achieve sustainability in managed ecosystems we must understand management impacts on soil processes and clarify the regulatory role of the microbial community on these processes. Crop rotation and organic management practices are thought to have positive impacts on the microbial biomass; however, the specific impacts of crop rotation organic management on soil microbial ecology are largely unknown. The effect of organic management on soil microbial ecology was investigated using soils collected from the Rodale Institute Research Center's long-term Farming Systems Trial (FST) experiment. The FST, begun in 1981, included a manured and a cover cropped organic rotation and a conventionally managed grain based rotation. Soil respiration rates and13C-isotope fate in a companion study suggest that the biomass characteristics of the FST treatment soils were different in November 1991. However, direct measurement of the microbial community at this time using Phospholipid Fatty Acid Analysis (PLFA) did not identify statistically significant treatment based differences in soil biomass characteristics. Variability among the PLFA profiles of treatment replicates was as great as variability between farming systems. Treatment based trends were observed among selected PLFAs, particularly those present in large amounts, that were consistent with indirect biomass and biomass-dependent measures. Overall, PLFA profiles, soil respiration rates and13C-cycling suggested that the organic cover cropped soil had the Largest and most heterogeneous microbial population while the biomass of the organic-manure amended soil was the least heterogeneous, and the most metabolically active.
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References
Anderson J P E 1982 Soil Respiration.In Methods in Soil Analysis. Ed. A L Page, pp 831–834. American Society of Agronomy, Madison.
Anderson T H and Domsch K H 1989 Ratios of microbial biomass carbon to total organic carbon in arable soils. Soil Biol. Biochem. 21, 471–479.
Angers D A, Peasant A and Vigneux J 1992 Early croppin-induced changes in soil aggregation, organic matter, and microbial biomass. Soil Sci. Soc. Am. J. 56, 115–119.
Baldock J A, Oades J M, Vassallo A M and Wilson M A 1990 Solid-State CP/MAS 13C.N.M.R./analysis of bacterial and fungal cultures isolated from a soil incubated with glucose. Aust. J. Soil Res. 28, 213–225.
Baird B H and White D C 1985 Biomass and community structure of the abyssal microbiota determined from the ester-linked phospholipids recovered from Venezuela basin and Puerto Rico trench sediments.In Benthic Ecology and Sedimentary Processes of Venezuela Basin-Past and Present. Eds. DC Young and MD Richardson, 68, 217–231, Elsevier, Amsterdam.
Balkwill D L, Leach F R, Wilson J T, McNabb J F, White D C 1988 Equivalence of microbial biomass measures based on membrane lipid cell wall components, adenosine triphosphate, and direct counts in surface aquifers. Microbial Ecol. 16, 73–84.
Bligh E6 and Dyer E6 1959 Arapid method for total lipid extraction and purification. Can. J. Biochem. Phys. 37, 911–917.
Bobbie R J and White D C 1980 Characterization of benthic microbial community structure by high resolution gas chromatography of fatty acid methyl esters. Appl. Environ. Microbiol. 39, 1212–1222.
Bolin B 1977 Changes of land biota and their importance for the carbon cycle. Science 196, 613–615.
Bolton H J, Elliott L F and Papendick R I 1985 Soil microbial biomass and selected soil enzyme activities: effect of fertilization and cropping practices. Soil Biol. Biochem. 17, 297–302.
Bott T L and Kaplan L A 1985 Bacterial biomass, metabolic state, and activity in stream sediments: relation to environmental variables and multiple assay conditions. Appl. Environ. Microbiol. 50, 508–522.
Christie W W 1989 Gas chromatography and lipids. The Oily Press, Ary, Scotland.
Colwell R R, Brayton P R, Grimes D J, Rosazak D B, Huq S A and Palmer L M 1985 Viable non-culturableVibrio cholerae and related pathogens in the environment: Implications for release of genetically engineered microoganisms. Biotechnol. 3, 817–820.
Doran J W 1987 Microbial biomass and mineralizable nitrogen distributions in no-tillage and plowed soils. Biol. Fert. Soils. 5, 68–75.
Doran J W, Fraser D G, Culick M N and Liebhardt W C 1987 Influence of alternative and conventional agricultural management on soil microbial processes and nitrogen availability. Am. J. Alt. Agric. 2, 99–109.
Dowling N J E, Widdel F and White D C 1986 Phospholipid esterlinked fatty acid biomarkers of acetate oxidizing sulfate reducers and other sulfide forming bacteria. J. Gen. Micro. 132, 1815–1825.
Edwards CA, Brust 6E, Stinner BR and McCartney DA 1992 work in the United States on eh use of cropping patterns to promote natural enemies of pests. Aspects of Appl. Biol. 31, 139–148.
Elliott E T and Coleman D C 1988 Let the soil work for us. Ecol. Bull. 39, 23–32.
Federle T W 1986 Microbial distributions in soil- new techniques. Perspectives in Microbial Ecology, Proceedings Fourth International Symposium in Microbial Ecology. Ed. M. Meguser. pp 493–498. American Society of Microbiology, Washington D.C.
Findlay RH, King Gmand Walting L 1989 Efficacy of Phospholipid analysis in determining microbial biomass in sedemints. Appl. Environ. Microbiol. 55, 2888–2892.
Friedman D B 1993 Carbon, nitrogen and aggregate dynamics in low-input and reduced tillage cropping systems. M.S. Thesis. Cornell University, Ithica, NY.
Guckert J B, Anthworth C P, Nichols P D and White D C 1986 Phodspholipid ester-linked fatty acid profile changes during nutrient deprivation ofVibrio cholerae: increase in the cis/trans ratio and proportions of the cyclopropyl fatty acids. Appl. Environ. Microbiol. 52, 797–801.
Guckert J B, Antworth C P, Nichols P D and White D C 1985 Phospholipid, ester-linked fatty acid profiles as reproducible assays for changes in procaryotic community structure of estuarine sediments. FEMS Microbiol. Ecol. 31, 147–158.
Hanson J C, Johnson D M, Peters S E and Janke R R 1990 The profitability of sustainable agriculture in the Mid-Atlantic region-a case study between 1991–1981. Department of Agriculture and Resource Economics, Working Paper no. 90–12, University of Maryland.
Harris G H, Hesterman O B, Paul E A, Harris D, Peters S E and Janke R R 1994 Carbon and nitrogen mineralization, and the role of microbial biomass during long-term incubations of soils fertilized with legume green manure, animal manure, and inorganic N. Soil Biol. Biochem. (In preparation).
Hassink J, Voshaar J H, Nijhus E H and van Veen J A 1991 Dynamics of microbial populations of a reclaimed-plodder soil under a conventional and a reduced-input farming system. Soil Biol. Biochem. 23, 515–524.
Hedrick D B, Richards B, Jewell W, Guckert J B and White D C 1991 Disturbance, starvation and overfeeding stress detected by microbial lipid biomarkers in high-solids high yield methanogenic reactors. J. Indust. Microbiol. 8, 91–98.
Jenkinson D S and Powlson D S 1976 The effect of biocidal treatments on metabolism in soil. V.A. method for determining microbial biomass in soil. Soil Biol. Biochem. 8, 209–213.
Jenkinson D S and Ladd J N 1981 Microbial biomass in soil: measurement and turnover.In Soil Biochemistry. Eds. E A Paul and J N Ladd pp 415–471. Marcel Dekker, NY.
Kates M 1986 Techniques in Lipidology: Isolation, Analysis, and Identification of Lipids Elsevier, Amsterdam.
Kennedy A C and Petersen C R 1993 The effect of management practices on the soil microbial community. Agronomy Abstr. 1993 Annual Meeting. Cincinnati, OH. 253p.
Kroppenstedt R M 1985 Fatty acid and menaquinone analysis of actinomycetes and related organisms.In Chemical methods in bacterial systematics. Eds. M Goodfellow and DE. Minnikin pp 173–199. Academic Press, London.
Lal R 1991 Soil conservation and biodiversity.In The Biodiversity of Microorganisms and Invertibrates: Its Role in Sustainable Agriculture. Ed. D L. Hawksworth pp 89–104. Redwood Press, Melksham.
Liebhardt W C, Andrews R W, Culik M N, Harwood R R, Janke R R, Radke J K and Rieger-Schwartz S L 1989 Crop production during conversion from conventional to lowinput methods. Agron. J. 81, 150–159.
McGill W B, Cambell C A, Dormaar J F, Paul E A and Anderson E A 1981 Soil Organic Matter Losses. Agricultural land: our disappearing heritage. A Symposium, Edmonton, AB.
Nichols P D and White D C 1989 Accumulation of polybetahydroxybutyrate in a methane-enriched, halogenated hydrocarbon-degrading soil column: implications for microbial community structure and nutritional status. Hydrobiologia. 76, 369–377.
O'Leary W M and Wilkinson S G 1988 Gram-positive bacteria. In Microbial Lipids. Eds. C Rateledge and S G Wilkinson. pp 117–185. Academic Press, London.
Paul E A 1990 Soils as components and controllers of ecosystem processes.In Symposium of British Ecological Society. pp 353–374. Blackwell Scientific, Oxford.
Peters S E, Janke R R and Bohlke M 1992 Rodale's Farming Systems Trial 1986–1990. Rodale Institute, Kutztown.
Smith G A, Nickels J S, Kerger B D, Davis D, Collins S P, Wilson J T, McNabb J F and White D C 1986 Quantitative characterization of microbial biomass and community structure in subsurface material: a prokaryotic consortium responsive to organic contamination. Can. J. Microbiol. 32, 104–111.
Stewart W D P 1991 The importance to sustainable agriculture and biodiversity among invertebrates and microorganisms.In The Biodiversity of Microorganisms and Invertebrates: Its Role in Sustainable Agriculture. Ed. DL Hawksworth. pp 3–6, Redwood Press, Melksham.
Tunlid A and White D C 1992 Biochemical analyses of biomass, community structure, nutritional status, and metabolic activity of microbial communities in soil.In Soil Biochemistry. Eds. G Stotzky and J-M Bollag. pp 229–267. Marcel Dekken INC. NY.
Vandee mer J 1989 The ecology of Intercropping University of Cambrige Cambrige.
Vestal J R and White D C 1989 Lipid analysis in microbial ecology. Bioscience 39, 535–541. Wander M M 1993 The impact of organic and conventional management on soil organic matter characteristics: results from Rodale's farming system trial experiment. Ph.D. Dissertation. Ohio State University, Columbus, Oh.
Wander M M, Dudley B R, Traina S J, Kaufman D, and Stinner BR 1995 A 13C-NMR investigation of acetate fate in organic and conventionally managed soils to be submitted to Soil Sci. Soc. Am. J. (In preparation).
Werner M R 1988 Impact of conversion to organic agricultural practices on soil invertibrate ecosystems, Ph.D. Dissertation. SUNY College Environmental Science and Foresrtry, Syracuse, NY.
Whik DC, Davis WM, Nickels JS, King JD and Robbie RJ 1979 Determination of the sedimentarymicrobial biomass by extraction lipid phosphate. Oecologia. 40, 51–62.
Wilkinson S G 1988 Gram-negative bacteria.In Microbial Lipids. Eds. C Ratledge and S G Wilkinson. pp 299–457. Academic Press, London.
Winer B J 1971 Statistical Principles in Experimental Design. McGraw-Hill, NY. 208 p.
Zelles L, Bai Q Y, Beck T and Beese F 1992 Signature fatty acids in phospholipids and lipopolysaccharides as indicators of microbial biomass and comunity structure in agricultural soils. Soil. Biol. Biochem. 24, 317–323.
Zelles L and Bai Q Y 1993 Fractionation of fatty acids derived from soil lipids by solid phase extraction and their quantitative analysis by GC-MS. Soil Biol. Biochem. 25, 495–507.
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Present address: University of Illinois, 11025. Goodwin ave. Urbana, IL 61801, USA
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Wander, M.M., Hedrick, D.S., Kaufman, D. et al. The functional significance of the microbial biomass in organic and conventionally managed soils. Plant Soil 170, 87–97 (1995). https://doi.org/10.1007/BF02183057
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DOI: https://doi.org/10.1007/BF02183057