Abstract
In vivo cannulation of retinal blood vessels has not been reported but would be useful in exploring local vascular parameters and new treatment modalities. We developed a microsurgical technique for cannulating rabbit and cat retinal vessels, using glass micropipettes with curved shanks made from standard capillary tubing. The micropipette was held by a micromanipulator, which was fitted with a fiberoptic sleeve for intraocular illumination and for micropipette tip protection during insertion through the sclera. Direct aspiration from and injection into retinal blood vessels were possible.
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Aldrich MS, Sherman SA, Greenberg HS (1985) Cerebrovascular complications of streptokinase infusion. JAMA 253:1777–1779
Andreucci VE (1978) Manual of renal micropuncture. Idelson, Naples
Archer DB, Ernest TJ, Newell FW (1974) Classification of branch retinal vein obstruction. Trans Am Acad Ophthalmol Otolaryngol 78:148–165
Ashton N (1950) Injection of the retinal vascular system in the enucleated eye in diabetic retinopathy. Br J Ophthalmol 34:38–41
Baldwin DJ (1980) Dry beveling of micropipette electrodes. J Neurosci Methods 2:153–161
Brown KT, Flaming DG (1975) Instrumentation and technique for beveling fine micropipette electrodes. Brain Res 86:172–180
BVO Study Group (1984) Argon laser photocoagulation for macular edema in branch vein occlusion. Am J Ophthalmol 98:271–282
BVO Study Group (1986) Argon laser scatter photocoagulation for prevention of neovascular and vitreous hemorrhage in branch vein occlusion. JAMA 104:34–41
Green RW (1981) Central retinal vein occlusion: a prospective histopathologic study of 29 eyes in 28 cases. Trans Am Ophthalmol Soc 79:371–422
Hilton GF (1986) Planned elevation of intraocular pressure with temporary occlusion of the central retinal artery during retinal surgery. Arch Ophthalmol 104:975
Hyvarinen L (1967) Vascular structures of the rabbit retina. Acta Ophthalmol 45:852–861
Johannsson JW, Jay AWL, Rowland S (1977) Electronic measurement of red cell flow in micropipettes. Can J Physiol Pharmacol 55:72–76
de Juan E, Chandler DB, Tetsuo H, Machemer R (1986) Gliovascular architecture in the rabbit retina. Invest Ophthalmol Vis Sci 27:1602–1608
Kohner EM, Pettit JE, Hamilton AM, Bulpitt CJ, Dollery CT (1976) Streptokinase in central retinal vein occlusion: a controlled clinical trial. Br Med J 1: 550–553
Magargal LE, Brown GC, Augsburger JJ, Parrish RK II (1981) Neovascular glaucoma following central retinal vein obstruction. Ophthalmology 88:1095–1101
Murakami K, Ho PC, Trempe CL, Pruett RC (1983) Traction detachment of the macula after branch retinal vein occlusion. Ann Ophthalmol 15:760–765
Murta JN, Cunha-Vaz JG, Sabo CA, Laski ME, Mota CC (1986) Microperfusion studies of the retinal vessels and inhibition of fluorescein transport by Benemid. Invest Ophthal Vis Sci [Suppl] 27:308
Mutlu F, Leopold IH (1964) Structure of the retinal vascular system of the cat and rabbit. Am J Ophthalmol 57:804–814
Newell FW (1982) Ophthalmology, principles and concepts, 5th edn. Mosby, St Louis, p 291
Peduzzi M, Debbia A, Guerrieri F, Bolzani R (1986) Abnormal blood rheology in retinal vein occlusion. Graefe's Arch Clin Exp Ophthalmol 224:83–85
Spitznas M (1983) Motorized teleguided stereotactic micromanipulator for vitreous microsurgery. Arch Ophthalmol 101:623–630
Urbaniak J (1985) Hand clinics, microvascular surgery, vol 1, no 2. Saunders, Philadelphia
Vurek GC, Bennett CM, Jamison RL, Troy JL (1967) An air driven micropipette sharpener. J Appl Physiol 22:191–192
Wiederhielm CA, Woodbury JW, Kurk S, Rushmer RF (1964) Pulsatile pressures in the microcirculation of frog's messentary. Am J Physiol 207:173–176
Wise GN, Dollery CT, Henkind P (1971) The retinal circulation. Harper & Row, New York
Wolbarsht ML, Landers MB, Robinson CH, Hickingbotham D (1976) A micromanipulator for intraocular surgery. Ophthalmic Surg 7:51–54
Wormington CM, Jaeger HA (1983) A new microelectrode positioner for electrophysiology in the intact mammalian eye. J Neurosci Methods 9:295–300
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Supported by Duke University Core Grant (1 P30 EYO5722), Medical Research Funds from the Veterans Administration, a Fight for Sight Student Fellowship, c/o Mr. Bob Hope, Fight for Sight, Inc., New York, and the National Society to Prevent Blindness, New York
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Allf, B.E., de Juan, E. In vivo cannulation of retinal vessels. Graefe's Arch Clin Exp Ophthalmol 225, 221–225 (1987). https://doi.org/10.1007/BF02175453
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DOI: https://doi.org/10.1007/BF02175453