Summary
Gibberellin-like activity was found to be present in bleeding-sap obtained from root systems ofHelianthus annuus. The presence of activity was detected with a newly described simple and sensitive dwarf pea epicotyl assay, and by the barley endosperm and dwarf maize (Zea mays) tests.
The gibberellin-like substance, or substances, are acidic and ethyl acetate soluble, having anR f value of 0.6–0.7 in paper chromatography with isopropyl alcohol: ammonia: water (10∶1∶1 v/v).
The quantity of gibberellin-like activity produced by the roots was estimated at approximately 0.05 μg GA3-equivalents/plant/day.
Other evidence for gibberellin synthesis in roots is discussed, together with the possibility that shoot and root have significant inter-relationships with regard to the production and utilisation of other types of growth hormone.
Zusammenfassung
Am Blutungssaft des Wurzelsystems vonHelianthus annuus wurde ein gibberellinartiges Wirkungsvermögen festgestellt. Das Vorhandensein dieser Aktivität wurde mittels eines neubeschriebenen einfachen und empfindlichen Tests an Zwergerbsen-Epikotylen sowie mit dem Gerstenendosperm- und dem Zwergmais-Test entdeckt.
Die gibberellinartigen Stoffe haben Säurecharakter und sind in Äthylacetat löslich. IhrR f -Wert wurde papierchromatographisch mit Isopropanol: Ammoniak: Wasser=10∶1∶1 v/v als Laufmittel zu 0,6–0,7 ermittelt.
Das Maß der von der Wurzel produzierten gibberellinartigen Aktivität lag bei etwa 0,05 μg GH3-Äquivalenten pro Pflanze und Tag.
Andere Hinweise auf eine Gibberellinsynthese in Wurzeln werden besprochen und ferner die Möglichkeit, daß in Sproß und Wurzel eine signifikante Wechselbeziehung hinsichtlich der Produktion und Verwendung anderer Wuchsstoffarten besteht.
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References
Butcher, D. N. The presence of gibberellin in excised tomato roots. J. exp. Bot.14, 272–280 (1963).
Chailakhian, M. Kh.: Principles of ontogenesis and physiology of flowering in higher plants. Canad. J. Bot.39, 1817–1841 (1961).
Frankland, B. andP. F. Wareing: Changes in endogenous gibberellins in relation to chilling of dormant seeds. Nature (Lond.)194, 313–314 (1962).
Grossenbacher, K. A.: Autonomic cycle of rate of exudation of plants. Amer. J. Bot.26, 107–109 (1939).
Ivanoff, S. S.: Guttation injuries of plants. Bot. Rev.29, 202–229, (1963).
Lockhart, J. A.: Studies on the organ of production of the natural gibberellin factor in higher plants. Plant Physiol.32, 204–207 (1957).
Loeffler, J. E., andJ. van Overbeek: Kinin activity in coconut milk. Colloq. Internat. du Cent. de la Recherche Scientifique No. 123, Régulateurs naturels de la Croissance Végétale, pp. 77–82. 1964.
Nicholls, P. B., andL. G. Paleg: Barley endosperm bioassay for gibberellins. Nature (Lond.)199, 823–824 (1963).
Phillips, I. D. J.: Root-shoot hormone relations I. The importance of an aerated root system in the regulation of growth hormone levels inHelianthus annuus. Ann. Bot., N. S.28, 17–35 (1964a).
—: Root-shoot hormone relations II. Changes in endogenous auxin concentration produced by flooding of the root system inHelianthus annuus. Ann. Bot., N. S.28, 36–45 (1964b).
—, andP. F. Wareing: Studies in dormancy of sycamore I. Seasonal changes in the growth-substance content of the shoot. J exp. Bot.9, 350–364 (1958).
Skoog, F., T. C. Boyer, andK. A. Grossenbacher: Effects of auxin on rates, periodicity, and osmotic relations in exudation. Amer. J. Bot26, 749–759 (1938).
Went, F. W.: Specific factors other than auxin affecting growth and root formation. Plant Physiol.13, 55–80 (1938).
— Effect of the root system on tomato stem growth. Plant Physiol.18, 51–65 (1943).
—: The development of stems and leaves. Plant growth substances (ed.F. Skoog), p. 287–297. Madison: University of Wisconsin Press 1951.
Wheeler, A. W.: Changes in the leaf-growth substance in cotyledons and primary leaves during the growth of dwarf bean seedlings. J. exp. Bot.11, 222–229 (1960).
Zweig G., S. Yamaguchi, andG. W., Mason: Translocation of C14-gibberellin in red kidney bean, normal corn, and dwarf corn. Advanc. Chem. Ser.28, 122–134 (1961).
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Phillips, I.D.J., Jones, R.L. Gibberellin-like activity in bleeding-sap of root systems ofHelianthus annuus detected by a new dwarf pea epicotyl assay and other methods. Planta 63, 269–278 (1964). https://doi.org/10.1007/BF02089315
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DOI: https://doi.org/10.1007/BF02089315