Abstract
Lectins fromDioclea grandiflora (DG) andCanavalia brasiliensis (CB) were compared with Concanavalin A (ConA) for their ability to induce paw edema and peritoneal cell immigration in rats. ConA caused a slight edema with a peak at 1 h after injection, while DG or CB induced a pronounced and long-lasting edema that reached a maximum at about 6 h. Different antiinflammatory drugs partially inhibited the edema. α-d-glucose (GLU) partially blocked the edema caused by ConA and markedly inhibited that due to CB, but had no effect on the edema induced by DG. α-Methyl mannoside (α-MM) blocked the edema caused by DG and ConA, but did not affect that caused by CB. At doses much lower than those used to induce paw edema, the lectins promoted an intense accumulation of neutrophil and mononuclear cells in the rat peritoneal cavity. CB and DG were more potent than ConA, which also presented a different profile of cell immigration. GLU significantly inhibited leukocyte accumulation caused by all lectins. α-MM impaired ConA- and DG-induced cell immigration, but only partially inhibited CB. Thus, despite their physicochemical similarities with ConA, DG and CB have more powerful pro-inflammatory effects. This difference seems to be related to their sugar-binding properties. However, while ConA- and DG-induced effects were inhibited more by α-MM than by GLU, CB-induced effects were inhibited more by glucose.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
I. J. Goldstein and R. D. Poretz,Isolation, physicochemical characterization and carbohydrate-binding specificity of lectins. InThe Lectins. Properties, functions and applications in biology and medicine. (Eds. I. E. Liener, N. Sharon and I. J. Goldstein) pp. 35, Academic Press, London 1986.
H. Lis and Sharon,Biological properties of lectins. InThe Lectins. Properties, functions and applications in biology and medicine. (Eds. I.E. Liener, N. Sharon and I.J. Goldstein) pp. 266, Academic Press, London 1986.
S. Serke, A. Neubauer and A. Van Lessen,Binding of mitogenic plant lectins to human lymphocytes. Flow cytometric analysis. J. Immunol. Methods26, 121–122 (1990)
A. J. Norin and R. A. De Pinho,Rate and efficiency of complement-dependent phagocytosis in cytosolic and noncytosolic inflammatory macrophages. Immunology58, 561–568 (1986).
R. P. MacDermont, M. J. Bragdon, I. J. Kodner and M. J. Bertovich,Deficient cell-mediated cytotoxicity and hyporesponsiveness to interferon and mitogenic lectin activation by inflammatory bowel disease peripheral blood and intestinal mononuclear cells. Gastroenterology90, 6–11 (1986).
R. A. Moreira, A. C. H. Barros, J. C. Stewart and A. Pusztai,Isolation and characterization of a lectin from seeds of Dioclea grandiflora (Mart.). Planta158, 63–69 (1983)
R. A. Moreira and B. S. Cavada,Lectin from Canavalia brasiliensis (Mart.). Isolation, characterization and behavior during germination. Biol. Plantarum 26(2), 113–120 (1984).
M. Barral-Neto, S. B. Santos, A. Barral, L. I. M. Moreira, C. F. Santos, R. A. Moreira, J. T. A. Oliveira and B. S. Cavada,Human lymphocyte stimulation by Legume lectins from Diocleae tribe. J. Immunol. Invest. (in press).
M. Barral-Neto, A. Barral, S. B. Santos, L. I. M. Moreira, C. F. Santos, F. M. Ramiro, M. R. P. Texeira, R. A. Moreira, J. T. A. Oliveira and B. S. Cavada,Can lectins be used as immunostimulants? InProc. 2nd Brazilian Symp. on Lectins. (Ed. Universidade Federal do Ceara, Brazil) (in press).
M. Russo,Use of lectins for macrophage activation. InProc. 2nd Brazilian Symp. on Lectins (Ed. Universidade Federal do Ceara, Brazil) (in press).
S. H. Ferreira,A new method for measuring variations of rat paw volume. J. Pharm. Pharmacol.31, 648 (1979).
C. Barja-Fidalgo, C. R. Carlini, J. A. Guimaräes, C. A. Flores, F. Q. Cunha and S. H. Ferreira,Role of resident macrophages in canatoxin-induced in vivo neutrophil migration. Inflammation 16(1), 1–12 (1992).
M. M. Dale,The neutrophil leukocyte. InTextbook of Immunopharmacology. (Eds. M. M. Dale and J. C. Foreman) p. 37, Blackwell Scientific Publication, Oxford 1989.
P. Needleman, J. Turk, B. Jakcschik, A. R. Morrison and J. B. Leftowith,Arachidonic acid metabolism. Ann. Rev. Biochem.55, 69–102 (1986).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bento, C.A.M., Cavada, B.S., Oliveira, J.T.A. et al. Rat paw edema and leukocyte immigration induced by plant lectins. Agents and Actions 38 (Suppl 1), 48–54 (1993). https://doi.org/10.1007/BF02027213
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02027213