Abstract
Bacterial strains were randomly isolated from soil using three different media with glucose (TG), Tryptone Soya Broth (TTS), and succinate (TS) as carbon sources. Plate counts obtained were 12.0×107, 4.5 ×107, and 1.5×107 g−1 soil dry weight, respectively. The strains were characterized phenotypically by the API 20B test system. A cluster analysis of all isolates revealed 40 biotypes at 80% similarity, 23 in TG, 29 in TTS, and 27 in TS. Each of the 10 most common biotypes contained 10 to 2.5% of the isolates, and 17 biotypes contained one or two isolates. The common biotypes were unevenly distributed among the isolates from the different media. About 20% of the isolates from TG and TTS were unique for the particular medium, whereas among the isolates from TS, about 60% were unique. Thirty percent of the isolates belonged to biotypes that were common to all three populations. All media gave approximately the same high diversity measured as Shannon index and Equitability, indicating no direct correlation between plate count and diversity.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Buchanan RE, Gibbons NE (1974) Bergey's manual of determinative bacteriology, 8th ed. Williams and Wilkins, Baltimore
Buck JD (1982) Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993
Gamble TN, Betlach MR, Tiedje JM (1977) Numerically dominant denitrifying bacteria from world soils. Appl Environ Microbiol 33:926–939
Hanssen JF, Thingstad TF, Goksøyr J (1974) Evaluation of hyphal lengths and fungal biomass in soil by a membrane filter technique. OIKOS 7:102–107
Hoffman PS, Pine L, Bell S (1983) Production of Superoxide and hydrogen peroxide in medium used to cultureLegionella pneumophilia: Catalytic decomposition by charcoal. Appl Environ Microbiol 45:784–791
Jensen V (1968) The plate count technique. In: Gray TRG, Parkinson D (eds) The ecology of soil bacteria. University Press, Liverpool, pp 158–170
Lance GN, Williams WT (1967) A general theory of classifactory sorting strategies. 1. Hierarchical systems. Computer J 9:373–380
Lockhead AG, Burton MO (1956) Importance of soil extract for the enumeration and study of soil bacteria. Sixieme Congres de la Science du Sol Paris III, pp 157–161
Martin JK (1975) Comparison of agar media for counts of viable soil bacteria. Soil Biol Biochem 7:401–402
Martin SE, Flowers RS, Ordal ZJ (1976) Catalase: Its effect on microbial enumeration. Appl Environ Microbiol 32:731–734
Mills AL, Wassel RA (1980) Aspects of diversity measurement for microbial communities. Appl Environ Microbiol 40:578–586
Olsen RA, Bakken LR (1987) Viability of soil bacteria: Optimization of plate-counting technique and comparison between total counts and plate counts within different size groups. Microb Ecol 13:59–74
Olsson S, Söderström B, Nordbring-Hertz B (1984) A fast and simple method for classification of soil bacterial populations. Z Pflanzenernaehr Bodenk 147:198–202
Pielou EC (1966) The measurement of diversity in different types of biological collections. J Theoret Biol 13:131–144
Pochon J (1954) Manuel technique d'analyse microbiologique du sol. Masson et Cie., Paris
Ristroph JD, Hedlund KW, Allen RG (1980) Liquid medium for growth ofLegionella pneumophilia. J Clin Microbiol 11:19–21
Rosswall T (1971) List of media used in microbiological studies. International Biological Programme—Swedish Tundra Biome Project. Technical Report No. 8
Shannon CE (1948) A mathematical theory of communication. Bell Syst Technol 27:379–423
Sokal RR, Michener CD (1958) A statistical method for evaluating systematic relationships. Univ Kansas Sci Bull 38:1049–1438
Sundman V (1970) Four bacterial soil populations characterized and compared by a factor analytical method. Can J Microbiol 16:455–464
Tate RL III, Mills AL (1983) Cropping and the diversity and function of bacteria in Pahokee muck. Soil Boil Biochem 15:175–179
Thornton HG (1922) On the development of a standardised agar medium for counting soil bacteria, with especial regard to the repression of spreading colonies. Ann Appl Biol 9:241–274
Williams RT, Crawford RL (1983) Microbial diversity of Minnesota peatlands. Microb Ecol 9:201–214
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sørheim, R., Torsvik, V.L. & Goksøyr, J. Phenotypical divergences between populations of soil bacteria isolated on different media. Microb Ecol 17, 181–192 (1989). https://doi.org/10.1007/BF02011852
Issue Date:
DOI: https://doi.org/10.1007/BF02011852