Abstract
Initial experiment on the reactions of five Japanese cultivars of cucumber toColletotrichum orbiculare infection in the greenhouse revealed that cv Suyo and Gibai were susceptible and moderately susceptible, respectively, while cv Shogoin fushinari and Sagami hanjiro were resistant to infection byC. orbiculare; cv Ochiai fushinari was moderately resistant. The ability of 16 plant growth promoting fungi (some isolates belonged to species ofPhoma and some non-sporulating isolates) isolated from zoysiagrass rhizospheres to induce systemic resistance in the above five cucumber cultivars was tested by growing plants in potting medium infested with barley grain inocula of PGPF in the greenhouse. The second true leaves of 21-day-old plants were challenge inoculated withC. orbiculare and disease assessed. Nine, out of 16 isolates, caused significant reduction of disease caused byC. orbiculare in at least two cultivars.Phoma isolates (GS8-1 and GS8-2) and non-sporulating isolates (GU21-2, GU23-3, and GU24-3) significantly reduced the disease in all the five cultivars. The disease suppression in cucumber was due to the induction of systemic resistance, since the inducer(s) and the pathogen were separated spatially and that the inducer did not colonize aerial portions. The resistance induced by certain isolates in a susceptible cultivar was less than that in a resistant cultivar. Disease suppression caused by isolate GU21-2 was similar to theC. orbiculare induced control in certain cultivars. The average rate of expansion of lesion diameter on leaves due toC. orbiculare was slower due to induction with the selected plant growth promoting fungi compared to the uninduced control plants. Roots of four cultivars were colonized by only three isolates, however, roots of one cultivar (Suyo) was colonized by five isolates suggesting the cultivar-specific root colonization ability.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- cv:
-
cultivar(s)
- PGPF:
-
plant growth promoting fungal isolates
- PGPR:
-
plant growth promoting rhizobacteria
References
Caruso F and Kuc J (1979) Induced resistance of cucumber to anthracnose and angular leaf spot byPseudomonas lachrymans andColletotrichum lagenarium. Physiological Plant Pathology 14: 191–201
Dean RA and Kuc J (1986) Induced systemic protection in cucumber: Effects of inoculum density on symptom development caused byColletotrichum lagenarium in previously infected and uninfected plants. Phytopathology 76: 186–189
Descalzo RC, Rahe JE and Mauza B (1990) Comparative efficacy of induced resistance for selected diseases of greenhouse cucumber. Canadian Journal of Plant Pathology 12: 16–24
Gomez KA and Gomez, AA (1984) Statistical procedures for Agricultural research. 2 ed. J. Wiley & Sons, New York pp. 680
Hammerschimdt R, Acres S and Kuc J (1976) Protection of cucumber againstColletotrichum lagenarium andCladosporium cucumerinum. Phytopathology 66: 790–793
Hyakumachi M, Ichikawa M and Kageyama K (1992) Plant growth promoting fungi isolated from rhizosphere ofZoysia japonica. (Abstract) Annals of the Phytopathological Society of Japan 59: 72
Hyakumachi M, Ichikawa M, Hayakawa T, Kohara E and Kageyama K (1993a) Identity and frequency of occurrence of plant growth promoting fungi from rhizospheres of turfgrass and cultivated crops. (Abstract) 6th International Congress of Plant Pathology Montreal, Canada p. 270
Hyakumachi M, Takatsugi H, Ishihara H and Kageyama K (1993b) Potentiality of plant growth promoting fungi in disease suppression. (Abstract) 6th International Congress of Plant Pathology Montreal, Canada p. 270
Ishiba C, Tani T and Murata M (1981) Protection of cucumber against anthracnose by a hypovirulent strain ofFusarium oxysporum f. sp.cucumerinum. Annals of the Phytopathological Society of Japan 47: 352–359
Jenns A and Kuc J (1980) Characteristics of anthracnose resistance induced by localized infection with tobacco necrosis virus. Physiological Plant Pathology 17: 81–91
Kuc J (1990) Immunization for the control of plant disease. In Hornby D (ed) Biological Control of Soil-borne Plant Pathogens (pp. 355–374). CAB International, Wallingford, UK
Kuc J, Shockley G and Kearney K (1975) Protection of cucumber againstColletotrichum lagenarium byColletotrichum lagenarium. Physiological Plant Pathology 7: 195–199
Kuc J and Richmond S (1977) Aspects of protection of cucumber againstColletotrichum lagenarium byC. lagenarium.Phytopathology 67: 533–536
Kuc J and Strobel NE (1992) Induced resistance using pathogens and non-pathogens. In: Tjamos EC (ed) Biological Control of Plant Diseases (pp. 295–300). Plenum Press, New York
Maurhofer M, Hase C, Meuwly P, Metraux J-P and Defago G (1994) Induction of systemic resistance of tobacco to tobacco necrosis virus by the root-colonizingPseudomonas fluorescens strain CHA0: Influence of thegac A gene and of pyoverdine production. Phytopathology 84: 139–146
Meera MS (1994) Induction of systemic resistance in cucumber against anthracnose using plant growth promoting fungi. Ph.D. Thesis, Gifu University, Japan pp. 154
Meera MS, Shivanna MB, Kageyama K and Hyakumachi M (1993) Induction of systemic resistance in cucumber plants using turfgrass rhizosphere fungi. Annals of the Phytopathological Society of Japan 59: 279
Meera MS, Shivanna MB, Kageyama K and Hyakumachi M (1994a) Some aspects of induction of systemic resistance in cucumber against anthracnose using plant growth promoting fungi. Annals of the Phytopathological Society of Japan 60: 328
Meera MS, Shivanna MB, Kageyama K and Hyakumachi M (1994b) Plant growth promoting fungi from zoysiagrass rhizosphere as potential inducers of systemic resistance in cucumbers. Phytopathology 84: 1399–1406
Meera MS, Shivanna MB, Kageyama K and Hyakumachi M (1995) Persistance of induced systemic resistance in cucumber in relation to root colonization by plant growth promoting fungal isolates. Crop Protection 14: 123–130
Shivanna MB, Meera MS and Hyakumachi M (1994) Sterile fungi from zoysiagrass rhizosphere as plant growth promoters in spring wheat. Canadian Journal of Microbiology 40: 637–644
Wei G, Kloepper JW and Tuzun S (1991) Induction of systemic resistance of cucumber toColletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria. Phytotopathology 81: 1508–1512
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Meera, M.S., Shivanna, M.B., Kageyama, K. et al. Responses of cucumber cultivars to induction of systemic resistance against anthracnose by plant growth promoting fungi. Eur J Plant Pathol 101, 421–430 (1995). https://doi.org/10.1007/BF01874856
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01874856