Summary
In order to demonstrate the presence of a Ca2+-activated Cl−-channel in theNitellopsis plasmalemma, tonoplast-free cells were prepared and their intracellular Ca2+ concentration was modified by internal perfusion. An increase in the Ca2+ concentration caused a large Cl− efflux with a concomitant depolarization of the membrane potential. These changes were for the most part reversible. The critical Ca2+ concentration was about 4.0 μm. Neither the Cl− efflux nor the membrane depolarization showed a time-dependent inactivation. A Cl−-channel blocker, A-9-C (9-anthracenecarboxylic acid) reduced both the Cl− efflux and the magnitude of the membrane potential depolarization. A small increase in the intracellular Ca2+ concentration, which is caused by membrane excitation of tonoplast-free cells is not sufficient to activate this Ca2+-dependent Cl−-channel.
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Shiina, T., Tazawa, M. Ca2+-dependent Cl− efflux in tonoplast-free cells ofNitellopsis obtusa . J. Membrain Biol. 106, 135–139 (1988). https://doi.org/10.1007/BF01871395
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DOI: https://doi.org/10.1007/BF01871395