Summary
Various modes of substrate and enzyme addition were used to hydrolyze a 10% concentration (w/v) of steam-exploded, water-and-alkali extracted aspenwood withTrichoderma harzianum E58 cellulases. Although cellulose conversion was high (94–100%), enzyme recovery was low in all cases. Low enzyme recovery was due to a combination of thermal inactivation and adsorption of the cellulases onto the lignocellulosic residue. Enzyme recycle was not feasible as the activity of the recovered cellulases towards crystalline cellulose was low. However, the residual material from enzyme hydrolysis was a suitable carbon source for cellulase enzyme production byT. harzianum based on enzyme yield and hydrolytic potential. These residues could only be used up to a 1% substrate concentration, since at higher substrate loadings cellulase production was reduced, likely because of lignin inhibitors.
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Beldman, G., A.G.J. Voragen, F.M. Rombouts and W. Pilnik. 1988. Synergism in cellulose hydrolysis by endoglucanases and exoglucanases purified fromTrichoderma viride. Biotechnol. Bioeng. 31: 173–178.
Charnoglazov, V.M., O.V. Ermolova, and A.A. Klyosov. 1988. Adsorption of high-purity endo-1,4-β-glucanases fromTrichoderma reesei on components of lignocellulosic materials: cellulose, lignin, and xylan. Enzyme Microb. Technol. 10: 503–507.
Doppelbauer, R., H. Esterbauer, W. Steiner, R.M. Lafferty and H. Steinmuller. 1987. The use of lignocellulosic wastes for production of cellulase byTrichoderma reesei. Appl. Microbiol. Biotechnol. 26: 485–494.
Klyosov, A.A., O.V. Mitkevich and A.P. Sinitsyn 1986. Role of the activity and adsorption of cellulases in the efficiency of the enzymatic hydrolysis of amorphous and crystalline cellulose. Biochemistry 25: 540–542.
Lee, Y-H. and L.T. Fan. 1983. Kinetic studies of enzymatic hydrolysis of insoluble cellulose: (II). Analysis of extended hydrolysis times. Biotechnol. Bioeng. 25: 939–966.
Mandels, M., R. Andreotti and C. Roche. 1976. Measurement of saccharifying cellulase. Biotechnol. Bioeng. Symp. 6: 21–33.
Mes-Hartree, M., C.M. Hogan, Q. Nguyen, G. Louis-Seize and J.N. Saddler. 1988. Enzymatic conversion of lignocellulosic material to fuels. In: Sixth Canadian Bioenergy R and D Seminar. (Granger, C., ed.), pp. 352–354. Elsevier Applied Sciences Publisher, New York.
Mes-Hartree, M., C.M. Hogan and J.N. Saddler. 1987. Recycle of enzymes and substrate following enzymatic hydrolysis of steam-pretreated aspenwood. Biotechnol. Bioeng. 30: 558–564.
Mes-Hartree, M., C.M. Hogan and J.N. Saddler. 1988. Influence of growth substrate on production of cellulase enzymes byTrichoderma harzianum E58. Biotechnol. Bioeng. 31: 725–729.
Miller, G.L. 1959. Use of dinitrosalicylic reagent for the determination of reducing sugars. Anal. Chem. 31: 426–428.
Montenecourt, B.S. and D.E. Eveleigh. 1977. Preparation of mutants ofTrichoderma reesei with enhanced cellulase production. Appl. Environ. Microbiol. 34: 777–782.
Schuler, A.T. and Q. Nguyen. 1989. Bioconversion opportunities and strategies: a pragmatic approach. Forest Prod. J. 39: 33–38.
Scopes, R.K. 1987. In: Protein Purification: Principles and Practice, 2nd Edition (Cantor, C.R., ed.), p. 29, Springer-Verlag, New York.
Sutcliffe, R. and J.N. Saddler. 1986. The role of lignin in the adsorption of cellulases during enzymatic treatment of lignocellulosic material. Biotechnol. Bioeng. Symp. 17: 749–762.
Vallander, L. and K.-E. Eriksson. 1987. Enzyme recirculation in saccharification of lignocellulosic materials. Enzyme Microb. Technol. 9: 714–720.
Vohra, R.M., C.K. Shirkot, S. Dhawan and K.G. Gupta. 1980. Effect of lignin and some of its components on the production and activity of cellulase(s) byTrichoderma reesei. Biotechnol. Bioeng. 22: 1497–1500.
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Hogan, C.M., Mes-Hartree, M. Recycle of cellulases and the use of lignocellulosic residue for enzyme production after hydrolysis of steam-pretreated aspenwood. Journal of Industrial Microbiology 6, 253–261 (1990). https://doi.org/10.1007/BF01575870
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DOI: https://doi.org/10.1007/BF01575870