Summary
The effect of inhibition of cytoplasmic streaming on intercellular passage of carboxyfluorescein (CF) in staminal hairs ofS. purpurea was examined. Tip cells of staminal hairs were microinjected with buffered-CF. Cytoplasmic streaming was then inhibited by addition of KCN or NaN3 to the external bathing solution. In separate experiments, cytoplasmic streaming was inhibited by microinjection of cytochalasin D along with the buffered-CF. CF passage over a 5 minutes treatment period was monitored by video fluorescence microscopy and video intensity analysis. Cytoplasmic streaming ceased within 1 minute of inhibitor agent treatment, however, little change in the kinetics of intercellular passage was noted over the 5 minute experimental period. Th us, cytoplasmic streaming plays no major role in the regulation of intercellular passage of the hydrophilic, negatively charged molecule CF.
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The work is dedicated to professor Saal Zalik, Department of Plant Science, University of Alberta, on his 65th birthday.
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Tucker, E.B. Cytoplasmic streaming does not drive intercellular passage in staminal hairs ofSetcreasea purpurea . Protoplasma 137, 140–144 (1987). https://doi.org/10.1007/BF01281149
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DOI: https://doi.org/10.1007/BF01281149