Abstract
Two strains of biosurfactant-producing bacteria, identified asPseudomonas aeruginosa, were isolated from injection water and crude oil-associated water in Venezuelan oil fields. Both biosurfactants resembled rhamnolipids and produced stable emulsions of heavy and extra-heavy crude oils, reducing the surface tension of water from 72 to 28 dynes/cm. Tenso-active properties of the biosurfactants were not affected by pH, temperature, salinity or Ca2+ or Mg2+ at concentrations in excess of those found in many oil reservoirs in Venezuela.
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References
Chaplin, M.F. & Kennedy, J.F. 1987Carbohydrate Analysis, a Practical Approach. Oxford: IRL Press.
Cooper, D. & Zajic, J. 1980 Surface active compounds from microorganisms.Advances in Applied Microbiology26, 229–253.
Doudoroff, M. & Palleroni, N. 1984 Gram-negative aerobic rods and cocci. InBergey's Manual of Determinative Bacteriology, 8th edn, eds Buchanan, R.E. and Gibbons pp. 141–199. N.E. Baltimore: Williams and Wilkins, Co.
Gutnick, D. 1984 Biosurfactants and the oil industry.The World Biotechnology Report2, 645–652.
Itoh, S., Honda, H., Tomita, F. & Susuki, T. 1971 Rhamnolipids produced byPseudomonas aeruginosa grown on n-paraffin.Journal of Antibiotics24, 855–859.
Kosaric, N., Gray, N.C.C. & Cairns, W.L. 1987 Biotechnology and the surfactant industry. InBiosurfactants and Biotechnology, Surfactant Science Series, Vol. 25, eds Kosaric, N., Cairns, W.L. and Gray, N.C.C. pp. 1–19. New York: Marcel Dekker.
Lang, S. & Wagner, F. 1987 Structure and properties of biosurfactants. InBiosurfactants and Biotechnology, Surfactant Science Series, Vol. 25, eds Kosaric, N., Cairns, W. L. and Gray, N. C. C. pp. 21–45. New York: Marcel Dekker Inc.
Mattei, G. & Bertrand, J. 1985 Production of biosurfactant by a mixed bacteria population grown in continuous culture on crude oil.Biotechnology Bioengineering7, 217–222.
Pines, O. & Gutnick, D. 1981 Relationship between phage resistance and emulsan production, interaction of phages with the cell-surface ofAcinetobacter calcoaceticus.Archives of Microbiology130, 129–133.
Rambeloarisoa, E., Rontani, J., Giusti, G., Duvnjak, Z. & Bertrand, J. 1984 Degradation of crude oil by a mixed population of bacteria isolated from sea-surface foams.Marine Biology83, 69–81.
Reiling, H.E., Thanei-Wiss, U., Guerra-Santos, L.H., Hirt, R., Kappeli, O. & Fiechter, A. 1986 Pilot plant production of rhamnolipid biosurfactant byPseudomonas aeruginosa.Applied and Environmental Microbiology51, 985–989.
Robert, M., Mercade, M.E., Bosch, M.P., Parra, J.L., Espuny, M.J., Manresa, M.A. & Guinea, J. 1989 Effect of the carbon source on biosurfactant production byPseudomonas aeruginosa 44T1.Biotechnology Letters11, 871–874.
Rosenberg, E., Zuckerberg, A., Rubinovitz, C. & Gutnick, D. 1979 Emulsifier ofArthrobacter RAG-1: isolation and emulsifying properties.Applied and Environmental Microbiology37, 402–408.
Syldatk, C. & Wagner, F. 1987 Production of biosurfactants. InBiosurfactants and Biotechnology, Surfactant Science Series, Vol. 25, eds Kosaric, N., Cairns, W.L. and Gray, N.C.C. pp. 89–120. New York: Marcel Dekker Inc.
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Rocha, C., San-Blas, F., San-Blas, G. et al. Biosurfactant production by two isolates ofPseudomonas aeruginosa . World J Microbiol Biotechnol 8, 125–128 (1992). https://doi.org/10.1007/BF01195830
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DOI: https://doi.org/10.1007/BF01195830