Abstract
Transfer of resistance toHeterodera avenae, the cereal cyst nematode (CCN), by a “stepping-stone” procedure from the wild grassAegilops ventricosa to hexaploid wheat has been demonstrated. The number of nematodes per plant was lower, and reached a plateau much earlier, in the resistant introgression line H93-8 (1–2 nematodes per plant) than in the recipient H10-15 wheat (14–16 nematodes per plant). Necrosis (hypersensitive reaction) near the nematode, little cell fusion, and few, often degraded syncytia were observed in infested H93-8 roots, while abundant, well-formed syncytia were present in the susceptible H10-15 wheat. Line H93-8 was highly resistant to the two Spanish populations tested, as well as the four French races (Fr1-Fr4), and the British pathotype Hall, but was susceptible to the Swedish pathotypes HgI and HgIII. Resistance was inherited as though determined by a single quasi-dominant factor in the F2 generations resulting from crosses of H93-8 with H10-15 and with Loros, a resistant wheat carrying the geneCre1 (syn.Ccn1). The resistance gene in H93-8 (Cre2 orCcn2) is not allelic with respect to that in Loros. RFLPs and other markers, together with the cytogenetical evidence, indicate that theCre2 gene has been integrated into a wheat chromosome without affecting its meiotic pairing ability. Introduction ofCre2 by backcrossing into a commercial wheat backgroud increases grain yield when under challenge by the nematode and is not detrimental in the absence of infestation.
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Andersen S, Andersen K (1982) Suggestions for determination and terminology of pathotypes and genes for resistance in cyst-forming nematodes, especiallyHeterodera avenae. EPPO Bull 12:379–386
Asiedu R, Fisher JM, Driscoll CJ (1990) Resistance toHeterodera avenae in the rye genome of triticale. Theor Appl Genet 79:331–336
Brown JAM (1973) Cereal cyst nematode. Comparative resistance in wheat and progress towards alien-resistance transfer. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp, Columbia, pp 1–7
Delibes A, García-Olmedo F (1973) Biochemical evidence of gene transfer from the Mv genome ofAegilops ventricosa to hexaploid wheat. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp, Columbia, pp 161–165
Delibes A, Sanchez-Monge R, Garcia-Olmedo F (1977) Bio-chemical and cytological studies of genetic transfer from the Mv genome ofAe. ventricosa into hexaploid wheat. A progress report. In: Sanchez-Monge E, García-Olmedo F (eds) Proc 8th Congress of EUCARPIA “Interspecific hybridization in plant breeding”, Madrid, pp 81–89
Dosba F, Cauderon Y (1972) A new interspecific hybridTriticum aestivum ssp vulgare ×Aegilops ventricosa. Wheat Inf Service 35:22–24
Dosba F, Rivoal R (1981) Les lignées d'addition blé-Aegilops ventricosa. II. Étude de leur comportement vis-á-vis d'Heterodera avenue. Agronomie 1:559–564
Dosba F, Doussinault G, Rivoal R (1978) Extraction, identification and utilization of the addition linesT. aestivum-Ae. ventricosa. In: Ramanujan S (ed) Proc 5th Int Wheat Genet Symp, New Delhi, pp 332–337
Doussinault G, Delibes A, Sanchez-Monge R, García-Olmedo F (1983) Transfer of a dominant gene for resistance to eyespot disease from a wild grass to hexaploid wheat. Nature, 303:698–700
Eastwood RF, Lagudah ES, Appels R, Hannah M, Kollmorgen JF (1991)Triticum tauschii: a novel source of resistance to cereal cyst nematode (Heterodera avenae). Aust J Agri Res 42:69–77
Ferris VR, Faghihi J, Ireholm A, Ferris JM (1989) Two-dimensional protein patterns of cereal cyst nematodes. Phytopathology 79:927–933
García-Olmedo F, Delibes A, Sanchez-Monge R (1984) Transfer of resistance to eyespot disease fromAegilops ventricosa to wheat. Vortr Pflanzanzüchtg 6:156–158
Koebner RMD, Martin PK (1989) Chromosomal control of aminopeptidases of wheats and its close relatives. Theor Appl Genet 78:657–664
Mena M, Orellana J, Lopez-Braña I, García-Olmedo F, Delibes A (1993) Characterization of wheat/Aegilops ventricosa introgression and addition lines with respect to the Mv genome. Theor Appl Genet 86:197–204
O'Brien PC, Fisher JM, Rathjen AJ (1980) Inheritance of resistance in two wheat cultivars to an Australian population ofHeterodera avenae. Nematologica 26:69–74
Rivoal R (1977) Identification des races biologiques du nematode a kystes des céreales,Heterodera avenae Woll., en France. Ann Zool Ecol Anim 9:261–272
Rivoal R, Dosba F, Jahier J, Pierre JS (1986) Les lignées d'addition blé-Aegilops ventricosa Tausch. IV. Etude de la localisation chromosomique de la résistance à l'égard d'Heterodera avenae. Agronomie 6:143–148
Sanchez A, Zancada MC (1987) Characterization ofHeterodera avenae pathotypes from Spain. Nematologica 33:55–60
Slootmaker LAJ, Lange W, Jochemsem G, Schepers J (1974) Monosomic analysis in bread wheat of resistance to cereal root eelworm. Euphytica 23:497–503
Trudgill DL (1991) Resistance to and tolerance of plant parasitic nematodes in plants. Annu Rev Phytopathol 29:167–192
Valdeolivas A, Romero MD (1990) Morphometric relationships of some members of theHeterodera avenae complex (Nematoda: Heteroderidae). Nematologica 36:292–303
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Delibes, A., Romero, D., Aguaded, S. et al. Resistance to the cereal cyst nematode (Heterodera avenae Woll.) transferred from the wild grassAegilops ventricosa to hexaploid wheat by a “stepping-stone” procedure. Theoret. Appl. Genetics 87, 402–408 (1993). https://doi.org/10.1007/BF01184930
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DOI: https://doi.org/10.1007/BF01184930