Abstract
Hybrid cell lines secreting monoclonal antibodies against mammalian neurofilaments have been prepared. An improved protocol for the preparation of neurofilaments and methods for the identification and isolation of such hybridomas are presented. The antibodies produced specifically stain only neuronal cell types in both cerebellar sections and culture.
Similar content being viewed by others
References
Anderton BH (1981)J. Muscle Res. and Cell Mo tility, in press.
Anderton BH, Ayers M, & Thorpe RC (1978)FEBS Letters 96, 159–163.
Anderton BH, Thorpe RC, Cohen J, Selvendran S, & Woodhams P (1980)J. Neurocytol. 9, 835–844.
Bennett GS, Fellini SA, Croop JM, Otto JJ, Bryan J, & Holtzer H (1978a)Proc. Natl. Acad. Sci. U.S.A. 75, 4364–4368.
Bennett GS, Fellini SA, & Holtzer H (1978b)Differentiation 12, 71–82.
Blose SH, Shelanski M, & Chacko S (1977)Proc. Natl. Acad. Sci. U.S.A. 74, 662–665.
Brown JP, Temerius JD, & Hellstrom I (1979)J. Imm. Methods 31, 201–209.
Dahl D (1980)FEBS Letters 111, 152–156.
Fellini SA, Bennett GS, Toyama Y, & Holtzer H (1978)Differentiation 12, 59–69.
Hoffman PN & Lasek RJ (1975)J. Cell. Biol. 66, 351–366.
Hudson L & Hay FC (1980)Practical Immunology, Blackwells, London.
Jacobs M, Choo F, & Thomas C (1980)Cell. Biol. Int. Rep. 4, 778.
Laemmli UK (1970)Nature (Lond.) 227, 680–685.
Lazarides E (1980)Nature (Lond.) 283, 249–256.
Liem RKH, Yen SH, Salomon GD, & Shelanski ML (1978)J. Cell Biol. 79, 637–645.
Micko S & Schlaepfer WW (1978)J. Neurochem. 30, 1041–1049.
Milstein C (1980)Scientific American 243, 56–106.
Thorpe RC, Delacourte A, & Anderton BH (1979a)FEBS Letters 181, 275–284.
Thorpe RC, Delacourte A, Ayers M, Bullock C, & Anderton BH (1979b)Biochem J. 181, 275–284.
Wang C, Asai DJ, & Lazarides E (1980)Proc. Natl. Acad. Sci. U.S.A. 77, 1541–1545.
Wood JN, Anderton BH, & Thorpe RC (1980)Biochem. Soc. Trans. 8, 615–616.
Yen SH & Fields KL (1981)J. Cell. Biol. 88, 115–126.
Yen SH, Dahl D, Schachner M, & Shelanski MC (1976)Proc. Natl. Acad. Sci. U.S.A. 73, 529–533.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wood, J.N., Anderton, B.H. Monoclonal antibodies to mammalian neurofilaments. Biosci Rep 1, 263–268 (1981). https://doi.org/10.1007/BF01114913
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01114913