Zusammenfassung
Eine einfache und schnelle Methode für die Extraktion, Derivatisierung und Bestimmung von Putrescin, Cadaverin, Histamin, Spermidin und Spermin als 5-Dimethylaminonaphthalin-1-sulfonyl-Derivate mit Hilfe der Hochdruck-Flüssigchromatographie (HPLC).
Die Amine werden mit einer wäßrigen Trichloressigsäure-Lösung aus dem Fisch extrahiert und mit Hilfe von 5-Dimethylaminonaphthalin-1-sulfonyl (Dansylchloride) derivatisiert. Die Reaktionsmischung wird auf eine RP-8-Säule injiziert und durch Gradientelution getrennt. Die beschriebene Methode bring einen beträchtlichen Zeitgewinn und eine Verringerung der Analysenkosten.
Summary
A simple and rapid method is described for the extraction, derivatization and subsequent determination by means of high pressure liquid chromatography of putrescine, cadaverine, histamine, spermidine and spermine as their 5-dimethylaminonaphthalene-1-sulphonyl derivatives.
The amines are extracted from the fish material by an aqueous trichloroacetic acid solution and derivatized by means of 5-dimethylaminonaphthalene-1-sulphonylchloride (dansyl chloride). The reaction mixture is then injected on a RP-8 column using gradient elution to separate the amine derivatives. Use of the method results in a considerable saving with respect to both time and costs.
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Rosier, J., Van Peteghem, C. A screening method for the simultaneous determination of putrescine, cadaverine, histamine, spermidine and spermine in fish by means of high pressure liquid chromatography of their 5-dimethylaminonaphthalene-l-sulphonyl derivatives. Z Lebensm Unters Forch 186, 25–28 (1988). https://doi.org/10.1007/BF01027175
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DOI: https://doi.org/10.1007/BF01027175