Summary
The enzymatic synthesis of a peptide compound was carried out successfully in homogeneous organic solvent.
Solid Thermolysin was found to catalyze the synthetic reaction of N-benzyloxycarbonyl-L-aspartyl-L-phenylalanine methyl ester (Z-APM; a precursor of sweetner Aspartame) from N-benzyloxycarbonyl-L-aspartic acid (Z-L-Asp) and L-phenylalanine methyl ester (L-PheOMe) in a 98 percent organic medium (ethylacetate∶benzene∶methanol∶water=50∶29∶19∶2). The dissolution of enzyme was not observed. The optimal pH shifted to acidic side by 1.0 pH unit, compared with that in aqueous medium. The enzymatic activity of solid thermolysin with an average size of 3.4×9.5 μm was determined to be 0.18 μmoles-product/(mg-solid)·h under the initial concentrations of L-PheOMe of 0.1M and Z-L-Asp of 0.05M, and at pH 6.0 and 40°C.
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References
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Ooshima, H., Mori, H. & Harano, Y. Synthesis of aspartame precursor by solid thermolysin in organic solvent. Biotechnol Lett 7, 789–792 (1985). https://doi.org/10.1007/BF01025555
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DOI: https://doi.org/10.1007/BF01025555