Summary
The optimum pH for both the rate of production and yield of hyaluronic acid (HA) byStreptococcus zooepidemicus from glucose medium was 6.7±0.2 under anaerobic conditions. High agitation rates (600 rpm) gave superior results compared to 300 rpm. Aeration of the culture (0.3 VVM) improved the HA yield, but not the rate of production and lead to some acetate and CO2 being formed, in addition to lactate and HA.
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References
Akasaka, H., Susumu, S., Yanagi, M., Fukushima, S., and Mitsui, T. (1988).J. Soc. Cosmet. Chem. Japan 22, 35–42.
Atlas, R.M. (1988).Microbiology, Fundamentals and Applications. NY: MacMillan.
Biotechnology General Corp. (1986).Patent WO 8604355.
Johns, M.R., and Stuart, D.M. (1991).J. Indust. Microbiol. 8, 23–28.
Kendall, F.E., Heidelberger, M. and Dawson, M.H. (1937).J. Biol. Chem. 118, 61–69.
Kery, V., Orvisky, E., and Stancikova, M. (1992).Carbohyd. Polymers 17, 227–230.
Stoolmiller, A.C., and Dorfman, A. (1969).J. Biol. Chem. 244, 236–246.
Terzaghi, B.E., and Sandine, W.E. (1975).Appl. Microbiol. 29, 807–813.
Van Brunt, J. (1986).Bio/Technology 4, 780–782.
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Johns, M.R., Goh, LT. & Oeggerli, A. Effect of pH, agitation and aeration on hyaluronic acid production byStreptococcus zooepidemicus . Biotechnol Lett 16, 507–512 (1994). https://doi.org/10.1007/BF01023334
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DOI: https://doi.org/10.1007/BF01023334